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Antimicrobial Agents and Chemotherapy, August 2000, p. 2077-2080, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bactericidal Activity of Gentamicin against Enterococcus faecalis In Vitro and In Vivo

Agnès Lefort,1 Michel Arthur,2 Louis Garry,1 Claude Carbon,1 Patrice Courvalin,2 and Bruno Fantin1,*

Institut National de la Santé et de la Recherche Médicale, EMI 9933, Hôpital Bichat-Claude Bernard,1 and Unité des Agents Antibactériens, Institut Pasteur,2 Paris, France

Received 3 December 1999/Returned for modification 26 February 2000/Accepted 10 May 2000

The activity of gentamicin at various concentrations against two strains of Enterococcus faecalis was investigated in vitro and in a rabbit model of aortic endocarditis. In vitro, gentamicin at 0.5 to 4 times the MIC failed to reduce the number of bacteria at 24 h. Rabbit or human serum dramatically increased gentamicin activity, leading to a >= 3-log10 CFU/ml decrease in bacterial counts when the drug concentration exceeded the MIC. Susceptibility testing in the presence of serum was predictive of in vivo activity, since gentamicin alone significantly reduced the number of surviving bacteria in the vegetations if the peak-to-MIC ratio was greater than 1. However, gentamicin selected resistant mutants in rabbits. The intrinsic activity of gentamicin should be taken into account in evaluation of combinations of gentamicin and cell wall-active agents against enterococci.

* Corresponding author. Mailing address: Service de Médecine Interne, Hôpital Beaujon, 100, Boulevard du Général Leclerc, 92118 Clichy Cedex, France. Phone: (33) (1) 40 87 58 90. Fax: (33) (1) 40 87 54 95. E-mail: bruno.fantin{at}bjn.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, August 2000, p. 2077-2080, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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