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Antimicrobial Agents and Chemotherapy, August 2000, p. 2207-2210, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mechanism of Metronidazole Resistance in Helicobacter pylori: Comparison of the rdxA Gene Sequences in 30 Strains

Nadia Maggi Solcà, Marco Valerio Bernasconi, and Jean-Claude Piffaretti*

Istituto Cantonale Batteriosierologico, 6904 Lugano, Switzerland

Received 23 September 1999/Returned for modification 11 January 2000/Accepted 18 May 2000

The rdxA gene of 30 independently isolated Helicobacter pylori strains was sequenced. A comparison of the rdxA sequences revealed a higher percentage of amino acid substitutions in the corresponding protein than in other housekeeping genes. Out of 122 point mutations, 41 were missense and 4 were nonsense. A resistant strain with a nucleotide insertion in the rdxA sequence was also found. With the exception of the point mutations and the insertion generating a stop signal, no particular nucleotide mutation or amino acid substitution could be associated to metronidazole resistance. Moreover, phylogenetic analysis of the 30 nucleotide sequences did not demonstrate specific clusters associated with the resistance phenotype.

* Corresponding author. Mailing address: Istituto Cantonale Batteriosierologico, Via Ospedale 6, 6904 Lugano, Switzerland. Phone: 41 91 923 25 22. Fax: 41 91 922 09 93. E-mail: jean-claude.piffaretti{at}ti.ch.

Antimicrobial Agents and Chemotherapy, August 2000, p. 2207-2210, Vol. 44, No. 8
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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