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Antimicrobial Agents and Chemotherapy, September 2000, p. 2271-2275, Vol. 44, No. 9
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Characterization of a Variant of vga(A)
Conferring Resistance to Streptogramin A and Related
Compounds
Julien
Haroche,1
Jeanine
Allignet,1
Carmen
Buchrieser,2 and
Névine
El Solh1,*
Unité des Staphylocoques, National
Reference Center for Staphylococci,1 and
Laboratoire de Génomique des Micro-Organismes
Pathogènes,2 Institut Pasteur, 75724 Paris
Cedex 15, France
Received 29 November 1999/Returned for modification 10 March
2000/Accepted 23 June 2000
A variant of the vga(A) gene (1,575 bp), encoding an
ATP-binding cassette protein conferring resistance to streptogramin A and related antibiotics, was cloned from the chromosome of a
Staphylococcus aureus clinical isolate and sequenced. The
sequence of the variant was similar to that of the vga(A)
gene (83.2% identity). However, the G+C content of the variant
(35.6%) was higher than that of vga(A) (29%) and there
was no cross hybridization between vga(A) and the variant
at high stringency (
60°C), the highest temperature at which a
signal was detected being 55°C. Unlike previous reports for
vga(A) and vga(B), the variant of
vga(A) may be present in multiple copies in the genome.
These copies are chromosomal in some isolates and both chromosomal and
plasmid-borne in others. Nucleotide sequences hybridizing at 65°C
with the vga(A) variant were found in all the
staphylococcal strains harboring plasmids carrying both
vga(B) and vat(B), which also encode resistance to streptogramin A.
*
Corresponding author. Mailing address: Unité des
Staphylocoques, National Reference Center for Staphylococci,
Institut Pasteur, 75724 Paris Cedex 15, France. Phone: (33) 01 45 68 83 63. Fax: (33) 01 40 61 31 63. E-mail: nelsolh{at}pasteur.fr.
Antimicrobial Agents and Chemotherapy, September 2000, p. 2271-2275, Vol. 44, No. 9
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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