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Antimicrobial Agents and Chemotherapy, September 2000, p. 2549-2553, Vol. 44, No. 9
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of FOX-4, a New AmpC-Type Plasmid-Mediated beta -Lactamase from an Escherichia coli Strain Isolated in Spain

Germán Bou,1,dagger Antonio Oliver,1 Mar Ojeda,2 Carmelo Monzón,2 and Jesús Martínez-Beltrán1,*

Servicio de Microbiología, Hospital Ramón y Cajal, 28034 Madrid,1 and Servicio de Microbiología, Hospital Insular, Las Palmas de Gran Canaria,2 Spain

Received 8 October 1999/Returned for modification 28 February 2000/Accepted 9 June 2000

A clinical strain of Escherichia coli (Ec GCE) displayed resistance to cefoxitin, cefotetan, cefotaxime, and ceftazidime. Susceptibility was not restored by the addition of clavulanic acid. Two beta -lactamases with apparent pIs of 5.4 and 6.4 were identified; the beta -lactamase with a pI of 6.4 was transferred by conjugation and associated with a 40-kb plasmid. Analysis of the nucleotide sequence showed a new ampC beta -lactamase gene that is closely related to those encoding the FOX-3, FOX-2, and FOX-1 beta -lactamases but whose product has four novel amino acid mutations, at positions 11 (Mright-arrowT), 43 (Aright-arrowE), 233 (Vright-arrowA), and 280 (Yright-arrowH). This first cephamycinase from Spain was named FOX-4.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Ramón y Cajal, Carretera Colmenar Viejo km. 9.100, 28034 Madrid, Spain. Phone: (91) 3368082. Fax: (91) 3368809. E-mail: jmtzbeltran{at}hrc.insalud.es.

dagger Present address: Department of Immunology, Mayo Clinic, Rochester, MN 55905.

Antimicrobial Agents and Chemotherapy, September 2000, p. 2549-2553, Vol. 44, No. 9
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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