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Antimicrobial Agents and Chemotherapy, September 2000, p. 2549-2553, Vol. 44, No. 9
Servicio de Microbiología, Hospital
Ramón y Cajal, 28034 Madrid,1 and
Servicio de Microbiología, Hospital Insular, Las
Palmas de Gran Canaria,2 Spain
Received 8 October 1999/Returned for modification 28 February
2000/Accepted 9 June 2000
A clinical strain of Escherichia coli (Ec GCE)
displayed resistance to cefoxitin, cefotetan, cefotaxime, and
ceftazidime. Susceptibility was not restored by the addition of
clavulanic acid. Two
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Molecular Characterization of FOX-4, a New
AmpC-Type Plasmid-Mediated
-Lactamase from an Escherichia
coli Strain Isolated in Spain

-lactamases with apparent pIs of 5.4 and 6.4 were identified; the
-lactamase with a pI of 6.4 was transferred by
conjugation and associated with a 40-kb plasmid. Analysis of the
nucleotide sequence showed a new ampC
-lactamase gene
that is closely related to those encoding the FOX-3, FOX-2, and FOX-1
-lactamases but whose product has four novel amino acid mutations,
at positions 11 (M
T), 43 (A
E), 233 (V
A), and 280 (Y
H). This
first cephamycinase from Spain was named FOX-4.
*
Corresponding author. Mailing address: Servicio de
Microbiología, Hospital Ramón y Cajal, Carretera Colmenar
Viejo km. 9.100, 28034 Madrid, Spain. Phone: (91) 3368082. Fax: (91)
3368809. E-mail: jmtzbeltran{at}hrc.insalud.es.
Present address: Department of Immunology, Mayo Clinic,
Rochester, MN 55905.
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