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Antimicrobial Agents and Chemotherapy, January 2001, p. 124-128, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.124-128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Studies of In Vitro Activities of Voriconazole and Itraconazole against Aspergillus Hyphae Using Viability Staining

Cornelia Lass-Flörl,1,* Markus Nagl,1 Cornelia Speth,1 Hanno Ulmer,2 Manfred P. Dierich,1 and Reinhard Würzner1

Department of Hygiene and Social Medicine1 and Department of Biostatistics and Documentation,2 University of Innsbruck, Innsbruck, Austria

Received 15 June 2000/Returned for modification 17 July 2000/Accepted 4 October 2000

The minimal fungicidal concentrations (MFCs) of voriconazole and itraconazole for five clinical isolates each of Aspergillus terreus, Aspergillus fumigatus, Aspergillus flavus, and Aspergillus niger were determined by a broth macrodilution method. Conidial suspensions as inocula were compared to hyphae as inocula since the invasive form of aspergillosis is manifested by the appearance of hyphal structures. In addition, cell viability staining with the dye FUN-1 was performed to assess time-dependent damage of hyphae exposed to various concentrations of the antifungal agents. With conidial inocula the MFC ranges of voriconazole were 0.5 to 4 µg/ml and those of itraconazole were 0.25 to 2 µg/ml, whereas the MFCs (2 to >16 µg/ml) with hyphal inocula were substantially higher (P < 0.01) for both itraconazole and voriconazole. Only minor differences between the tested antifungals were observed since 16 of 20 and 17 of 20 of the isolates of Aspergillus spp. tested appeared to be killed by voriconazole and itraconazole, respectively. The results of FUN-1 viability staining correlated closely to colony counts, but various time- and dose-dependent levels of viability of hyphae were also observed. In conclusion, our study demonstrates the importance of the type of inoculum used to test antifungals and the applicability of FUN-1 staining as a rapid and sensitive method for assaying the viability of hyphae.

* Corresponding author. Mailing address: Department of Hygiene and Social Medicine, Leopold-Franzens-University, Fritz Pregl-Str. 3, 6020 Innsbruck, Austria. Phone: 0043 512/507-3425. Fax: 0043 512/507-2870. E-mail: Cornelia.Lass-Floerl{at}uibk.ac.at.

Antimicrobial Agents and Chemotherapy, January 2001, p. 124-128, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.124-128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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