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Antimicrobial Agents and Chemotherapy, January 2001, p. 356-358, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.356-358.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of a Recombinant Strain of Mycobacterium avium Expressing beta -Galactosidase To Evaluate the Activities of Antimycobacterial Agents inside Macrophages

Giuseppantonio Maisetta, Giovanna Batoni,* Manuela Pardini, Antonella Boschi, Daria Bottai, Semih Esin, Mario Campa, and Sonia Senesi

Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, University of Pisa, Pisa, Italy

Received 30 May 2000/Returned for modification 6 July 2000/Accepted 25 October 2000

A reliable and low-cost method that enables rapid screening of the activity exerted by new antimicrobial agents on intracellularly growing Mycobacterium avium has been developed. To this aim, a recombinant (lacZ) strain of M. avium expressing the Escherichia coli beta -galactosidase gene was used to evaluate, in murine macrophages, the susceptibility of M. avium to common antimycobacterial agents. beta -Galactosidase levels, measured in the presence of each of the antibiotics tested, were closely correlated with the number of CFU recovered from the M. avium lacZ strain-infected macrophages.

* Corresponding author. Mailing address: Dipartimento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Via S. Zeno 35/39, 56127 Pisa, Italy. Phone: 39-050-836565. Fax: 39-050-836570. E-mail: batoni{at}biomed.unipi.it.

Antimicrobial Agents and Chemotherapy, January 2001, p. 356-358, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.356-358.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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