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Antimicrobial Agents and Chemotherapy, October 2001, p. 2691-2694, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2691-2694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Aminoglycoside Resistance Genes aph(2")-Ib and aac(6')-Im Detected Together in Strains of both Escherichia coli and Enterococcus faecium

Joseph W. Chow,1,2,* Vivek Kak,1,2 Il You,1 Susan J. Kao,1 Joanne Petrin,3 Don B. Clewell,4 Stephen A. Lerner,2 George H. Miller,3,dagger and Karen J. Shaw3,Dagger

Research and Medical Service, John D. Dingell VA Medical Center,1 and Division of Infectious Diseases, Wayne State University School of Medicine,2 Detroit, Michigan 48201-2097; Schering-Plough Research Institute, Kenilworth, New Jersey 070333; and Departments of Biologic and Materials Sciences and Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 481094

Received 22 January 2001/Returned for modification 28 March 2001/Accepted 27 June 2001

Escherichia coli SCH92111602 expresses an aminoglycoside resistance profile similar to that conferred by the aac(6')-Ie-aph(2")-Ia gene found in gram-positive cocci and was found to contain the aminoglycoside resistance genes aph(2")-Ib and aac(6')-Im (only 44 nucleotides apart). aph(2")-Ib had been reported previously in Enterococcus faecium SF11770. aac(6')-Im had not been detected previously in enterococci and was found to be present also 44 nucleotides downstream from aph(2")-Ib in E. faecium SF11770. aph(2")-Ib and aac(6')-Im are separate open reading frames, each with its own putative ribosome binding site, whereas aac(6')-Ie-aph(2")-Ia appears to be a fusion of two genes with just one start and one stop codon. The deduced AAC(6')-Im protein exhibits 56% identity and 80% similarity to the AAC(6')-Ie domain of the bifunctional enzyme AAC(6')-APH(2"). Our results document the existence of a member of the aph(2") family of genes in gram-negative bacteria and provide evidence suggesting the horizontal transfer of aph(2")-Ib and aac(6')-Im as a unit between gram-positive and gram-negative bacteria.

* Corresponding author. Mailing address: Medical Service, John D. Dingell VA Medical Center, 4646 John R, Detroit, MI 48201-1932. Phone: (313) 576-3310. Fax: (313) 576-1122. E-mail: JChow{at}wayne.edu.

dagger Present address: Microcide Pharmaceuticals, Mountain View, CA 94043.

Dagger Present address: RW Johnson Pharmaceutical Research Institute, San Diego, CA 92121.

Antimicrobial Agents and Chemotherapy, October 2001, p. 2691-2694, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2691-2694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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