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Antimicrobial Agents and Chemotherapy, October 2001, p. 2939-2942, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2939-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Antioxidant Functions Required for Insusceptibility of Saccharomyces cerevisiae to Tetracycline Antibiotics

Fiona E. Angrave and Simon V. Avery*

School of Life and Environmental Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom

Received 30 April 2001/Returned for modification 6 June 2001/Accepted 14 July 2001

Cu,Zn superoxide dismutase (Sod1) is required for insusceptibility of Saccharomyces cerevisiae to oxytetracycline (OTC). Here we report that Sod1 is also required for insusceptibility to doxycycline (DOX). Furthermore, among a range of antioxidant and redox balance mutants, mac1 and ctr1 deletion strains exhibited marked sensitization to OTC and DOX. Certain mutants exhibited a slight sensitivity to methacycline and minocycline. Addition of copper suppressed antibiotic sensitivity. Thus, intracellular copper as well as superoxide dismutase can be critical for eukaryotic tolerance of several tetracycline antibiotics.

* Corresponding author. Mailing address: School of Life and Environmental Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom. Phone: 44-115-9513315. Fax: 44-115-9513251. E-mail: simon.avery{at}nottingham.ac.uk.

Antimicrobial Agents and Chemotherapy, October 2001, p. 2939-2942, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2939-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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  • Marvin, M. E., Williams, P. H., Cashmore, A. M. (2003). The Candida albicansCTR1 gene encodes a functional copper transporter. Microbiology 149: 1461-1474 [Abstract] [Full Text]  
  • Blackburn, A. S., Avery, S. V. (2003). Genome-Wide Screening of Saccharomyces cerevisiae To Identify Genes Required for Antibiotic Insusceptibility of Eukaryotes. Antimicrob. Agents Chemother. 47: 676-681 [Abstract] [Full Text]  


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