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Antimicrobial Agents and Chemotherapy, October 2001, p. 2965-2968, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2965-2968.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Biochemical-Genetic Characterization of the Chromosomally Encoded Extended-Spectrum Class A beta -Lactamase from Rahnella aquatilis

Samuel Bellais, Laurent Poirel, Nicolas Fortineau, Jean W. Decousser, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France

Received 23 January 2001/Returned for modification 15 May 2001/Accepted 13 July 2001

From whole-cell DNA of a clinical isolate of the enterobacterial species Rahnella aquatilis, a beta -lactamase gene was cloned that encoded a chromosomally encoded Ambler class A enzyme, RAHN-1. RAHN-1, with a pI of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum beta -lactamase of chromosomal origin from Serratia fonticola and with the plasmid-mediated beta -lactamases CTX-M-2 and CTX-M-1, respectively. The hydrolysis spectrum of the clavulanic acid-inhibited RAHN-1 was expanded to cephalosporins such as cefuroxime, cefotaxime, and ceftriaxone, but not to ceftazidime. Its expression was not inducible.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, October 2001, p. 2965-2968, Vol. 45, No. 10
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.10.2965-2968.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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