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Antimicrobial Agents and Chemotherapy, November 2001, p. 2991-3000, Vol. 45, No. 11
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.11.2991-3000.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Molecular Analysis of Antibiotic Resistance Gene
Clusters in Vibrio cholerae O139 and O1 SXT
Constins
Bianca
Hochhut,1
Yasmin
Lotfi,1
Didier
Mazel,2
Shah M.
Faruque,3
Roger
Woodgate,4 and
Matthew
K.
Waldor1,*
Division of Geographic Medicine/Infectious Diseases, New
England Medical Center, Tufts University School of Medicine, and
Howard Hughes Medical Institute, Boston, Massachusetts
021111; Unité de Programmation
Moléculaire et Toxicologie Génétique, Institut
Pasteur, 75724 Paris Cedex 15, France2;
Molecular Genetics Laboratory, International Centre for
Diarrhoeal Disease Research, Bangladesh, Dhaka-1000,
Bangladesh3; and Section on DNA
Replication, Repair and Mutagenesis, National Institute of Child
Health and Human Development, National Institutes of Health,
Bethesda, Maryland 208924
Received 16 May 2001/Returned for modification 11 July
2001/Accepted 30 July 2001
Many recent Asian clinical Vibrio cholerae E1 Tor O1
and O139 isolates are resistant to the antibiotics sulfamethoxazole
(Su), trimethoprim (Tm), chloramphenicol (Cm), and streptomycin (Sm). The corresponding resistance genes are located on large conjugative elements (SXT constins) that are integrated into prfC on
the V. cholerae chromosome. We determined the DNA sequences
of the antibiotic resistance genes in the SXT constin in MO10, an O139
isolate. In SXTMO10, these genes are clustered within a
composite transposon-like structure found near the element's 5' end.
The genes conferring resistance to Cm (floR), Su
(sulII), and Sm (strA and strB)
correspond to previously described genes, whereas the gene conferring
resistance to Tm, designated dfr18, is novel. In some other
O139 isolates the antibiotic resistance gene cluster was found to be
deleted from the SXT-related constin. The El Tor O1 SXT constin,
SXTET, does not contain the same resistance genes as
SXTMO10. In this constin, the Tm resistance determinant was
located nearly 70 kbp away from the other resistance genes and found in
a novel type of integron that constitutes a fourth class of resistance integrons. These studies indicate that there is considerable flux in
the antibiotic resistance genes found in the SXT family of constins and
point to a model for the evolution of these related mobile elements.
*
Corresponding author. Mailing address: Division of
Geographic Medicine/Infectious Diseases, New England Medical Center and Tufts University School of Medicine, Box 041, 750 Washington St., Boston, MA 02111. Phone: (617) 636 7618. Fax: (617) 636 5292. E-mail: mwaldor{at}lifespan.org.
Antimicrobial Agents and Chemotherapy, November 2001, p. 2991-3000, Vol. 45, No. 11
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.11.2991-3000.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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