This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ma, L. Articles by Kovacs, J. A. Search for Related Content PubMed PubMed Citation Articles by Ma, L. Articles by Kovacs, J. A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2001, p. 3213-3215, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3213-3215.2001

Genetic Analysis of Multiple Loci Suggests that Mutations in the Pneumocystis carinii f. sp. hominis Dihydropteroate Synthase Gene Arose Independently in Multiple Strains

Liang Ma^* and Joseph A. Kovacs

Critical Care Medicine Department, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland

Received 9 April 2001/Returned for modification 16 May 2001/Accepted 30 July 2001

To determine if mutations in the dihydropteroate synthase (DHPS) gene of Pneumocystis carinii f. sp. hominis arose in a single strain that was subsequently widely disseminated, we examined four genomic regions of 22 P. carinii clinical isolates selected based on the absence or presence of mutations in the DHPS gene. By single-strand conformation polymorphism and DNA sequencing, we found varying genotypes for each of the four regions in isolates with DHPS mutations, suggesting that these mutations occurred independently in multiple strains of P. carinii. This suggests that exposure to sulfa will select for these mutations in diverse strains.

---

^* Corresponding author. Mailing address: Building 10, Room 7D43, National Institutes of Health, 10 Center Dr. MSC 1662, Bethesda, MD 20892-1662. Phone: (301) 435-2301. Fax: (301) 402-1213. E-mail: LMa{at}nih.gov

---

Antimicrobial Agents and Chemotherapy, November 2001, p. 3213-3215, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3213-3215.2001

This article has been cited by other articles:

• Iliades, P., Meshnick, S. R., Macreadie, I. G. (2004). Dihydropteroate Synthase Mutations in Pneumocystis jiroveci Can Affect Sulfamethoxazole Resistance in a Saccharomyces cerevisiae Model. Antimicrob. Agents Chemother. 48: 2617-2623 [Abstract] [Full Text]  
• Totet, A., Duwat, H., Magois, E., Jounieaux, V., Roux, P., Raccurt, C., Nevez, G. (2004). Similar genotypes of Pneumocystis jirovecii in different forms of Pneumocystis infection. Microbiology 150: 1173-1178 [Abstract] [Full Text]