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Antimicrobial Agents and Chemotherapy, November 2001, p. 3246-3249, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3246-3249.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Tetracycline Resistance Gene, tet(32), in the Clostridium-Related Human Colonic Anaerobe K10 and Its Transmission In Vitro to the Rumen Anaerobe Butyrivibrio fibrisolvens

Claire M. Melville, Karen P. Scott,* Derry K. Mercer, and Harry J. Flint

Rowett Research Institute, Bucksburn, Aberdeen, United Kingdom AB21 9SB

Received 15 February 2001/Returned for modification 26 April 2001/Accepted 14 August 2001

A novel tetracycline resistance gene, designated tet(32), which confers a high level of tetracycline resistance, was identified in the Clostridium-related human colonic anaerobe K10, which also carries tet(W). tet(32) was transmissible in vitro to the rumen anaerobe Butyrivibrio fibrisolvens 2221R. The predicted gene product of tet(32) has 76% amino acid identity with Tet(O). PCR amplification indicated that tet(32) is widely distributed in the ovine rumen and in porcine feces.

* Corresponding author. Mailing address: Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom. Phone: 44 (0) 1224 712751. Fax: 44 (0) 1224 716687. E-mail: kps{at}rri.sari.ac.uk.

Antimicrobial Agents and Chemotherapy, November 2001, p. 3246-3249, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3246-3249.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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