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Antimicrobial Agents and Chemotherapy, November 2001, p. 3256-3261, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3256-3261.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Congeners of SMAP29 Kill Ovine Pathogens and Induce Ultrastructural Damage in Bacterial Cells

V. C. Kalfa,1 H. P. Jia,2 R. A. Kunkle,1 P. B. McCray Jr.,2 B. F. Tack,3 and K. A. Brogden1,*

Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 50010,1 and Departments of Pediatrics2 and Microbiology,3 College of Medicine, The University of Iowa, Iowa City, Iowa 52242

Received 19 January 2001/Returned for modification 5 June 2001/Accepted 8 August 2001

SMAP29, an ovine cathelicidin, was systematically altered to create a family of 23 related peptides for MIC and minimum bactericidal concentration determinations. SMAP28, SMAP29, and a derivative of SMAP29 called ovispirin were all antimicrobial. However, many congeners of SMAP29 and ovispirin were not as active as the parent molecules. With immunoelectron microscopy, SMAP29 was seen on membranes and within the cytoplasm of Pseudomonas aeruginosa PAO1.

* Corresponding author. Mailing address: Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, 2300 Dayton Ave., P.O. Box 70, Ames, IA 50010. Phone: (515) 663-7534. Fax: (515) 663-8458. E-mail: kbrogden{at}nadc.ars.usda.gov.

Antimicrobial Agents and Chemotherapy, November 2001, p. 3256-3261, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3256-3261.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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