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Antimicrobial Agents and Chemotherapy, December 2001, p. 3341-3346, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3341-3346.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A MATE Family Multidrug Efflux Transporter Pumps out Fluoroquinolones in Bacteroides thetaiotaomicron

Shin Miyamae,1,2,3 Ohmi Ueda,2 Fuminobu Yoshimura,1,2 Jaiweon Hwang,1,dagger Yoshinobu Tanaka,3 and Hiroshi Nikaido1,*

Department of Molecular and Cell Biology, University of California, Berkeley, California,1 and Department of Microbiology2 and the First Department of Prosthodontics,3 School of Dentistry, Aichi-Gakuin University, Nagoya, Japan

Received 10 May 2001/Returned for modification 9 June 2001/Accepted 31 July 2001

We cloned a gene, bexA, that codes for a multidrug efflux transporter from the chromosomal DNA of Bacteroides thetaiotaomicron ATCC 29741 by using an Escherichia coli Delta acrAB Delta acrEF mutant as a host. Although the initial recombinant construct contained other open reading frames, the presence of bexA alone was sufficient to confer to the E. coli host elevated levels of resistance to norfloxacin, ciprofloxacin, and ethidium bromide. Disruption of bexA in B. thetaiotaomicron made the strain more susceptible to norfloxacin, ciprofloxacin, and ethidium bromide, showing that this gene is expressed in this organism and functions as a multidrug efflux pump. The deduced BexA protein sequence was homologous to the protein sequence of Vibrio parahaemolyticus NorM, a multidrug efflux transporter, and thus, BexA belongs to the multidrug and toxic compound extrusion (MATE) family.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Room 229, Stanley Hall, University of California, Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290. E-mail: nhiroshi{at}uclink4.berkeley.edu.

dagger Present address: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94735.

Antimicrobial Agents and Chemotherapy, December 2001, p. 3341-3346, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3341-3346.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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