Antimicrobial Agents and Chemotherapy, December 2001, p. 3595-3598, Vol. 45, No. 12
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.12.3595-3598.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
-Lactamase from Kluyvera
cryocrescens
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France
Received 26 February 2001/Returned for modification 23 June 2001/Accepted 28 August 2001
A chromosomally located
-lactamase gene, cloned and expressed in
Escherichia coli from a reference strain of the
enterobacterial species Kluyvera cryocrescens, encoded a
clavulanic acid-inhibited Ambler class A enzyme, KLUC-1, with a pI
value of 7.4. KLUC-1 shared 86% amino acid identity with a subgroup of
plasmid-mediated CTX-M-type extended-spectrum
-lactamases (CTX-M-1,
-3, -10, -11, and -12), the most closely related enzymes, and 77%
amino acid identity with KLUA-1 from Kluyvera ascorbata.
The substrate profile of KLUC-1 corresponded to that of CTX-M-type enzymes.
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