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Antimicrobial Agents and Chemotherapy, December 2001, p. 3625-3628, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3625-3628.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nonculture Prediction of Neisseria meningitidis Susceptibility to Penicillin

Aude Antignac, Jean-Michel Alonso, and Muhamed-Kheir Taha*

Unité des Neisseria, Centre National de Référence des Méningocoques, Institut Pasteur, 75724 Paris Cedex 15, France

Received 8 May 2001/Returned for modification 16 August 2001/Accepted 12 September 2001

We developed a nonculture method to predict the susceptibility of Neisseria meningitidis to penicillin G. The penA gene was amplified and submitted to restriction fragment length polymorphism analysis. This approach was first validated with a collection of 75 meningococcal strains of known phenotypes. It was next successfully applied to 29 clinical samples.

* Corresponding author. Mailing address: Unité des Neisseria, Centre National de Référence des Méningocoques, Institut Pasteur, 28 Rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 44 (0) 1 45 68 84 38. Fax: 44 (0) 1 45 68 83 38. E-mail: mktaha{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, December 2001, p. 3625-3628, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3625-3628.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.