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Antimicrobial Agents and Chemotherapy, December 2001, p. 3654-3656, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3654-3656.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of Green Fluorescent Protein as a Marker for Effects of Antileishmanial Compounds In Vitro

Sarah W. Kamau, Felix Grimm, and Adrian B. Hehl*

Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland

Received 20 March 2001/Returned for modification 29 June 2001/Accepted 27 August 2001

Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.

* Corresponding author. Mailing address: Institute of Parasitology, University of Zürich, Winterthurerstrasse 266a, 8057 Zürich, Switzerland. Phone: 41-1-635-8526. Fax: 41-1-635-8907. E-mail: ahehl{at}vetparas.unizh.ch.

Antimicrobial Agents and Chemotherapy, December 2001, p. 3654-3656, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3654-3656.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.