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Antimicrobial Agents and Chemotherapy, February 2001, p. 616-620, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.616-620.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nucleotide Sequence and Characterization of a Novel Cefotaxime-Hydrolyzing beta -Lactamase (CTX-M-10) Isolated in Spain

Antonio Oliver, José Claudio Pérez-Díaz, Teresa M. Coque, Fernando Baquero, and Rafael Cantón*

Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain

Received 19 May 2000/Returned for modification 21 August 2000/Accepted 28 October 2000

A cefotaxime-resistant, ceftazidime-susceptible Escherichia coli isolate was obtained from a patient with sepsis in 1997, from which a beta -lactamase with a pI of 8.1 was cloned. Cephaloridine and cefotaxime relative hydrolysis rates were 167 and 81, respectively (penicillin G rate = 100), whereas ceftazidime hydrolysis was not detected. The nucleotide sequence revealed a bla gene related to that coding for CTX-M-3. Despite 21 nucleotide substitutions, only 2 determined amino acid changes (Ala27Val and Arg38Gln). The amino acid sequence identity between this enzyme, designated CTX-M-10, and the chromosomal beta -lactamase of Kluyvera ascorbata was 81%.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Ramón y Cajal, Cta. Colmenar Km 9.1, Madrid 28034, Spain. Phone: 34 91 3368330. Fax: 34 91 3368809. E-mail: rcanton{at}hrc.insalud.es.

Antimicrobial Agents and Chemotherapy, February 2001, p. 616-620, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.616-620.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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