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Antimicrobial Agents and Chemotherapy, March 2001, p. 870-877, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.870-877.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Penicillin-Binding Proteins in Leptospira interrogans

Audrey Brenot,1 Daren Trott,2 Isabelle Saint Girons,*,1 and Richard Zuerner2

Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France,1 and Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, Iowa 500102

Received 7 July 2000/Returned for modification 30 September 2000/Accepted 8 December 2000

The Leptospira interrogans ponA and pbpB genes were isolated and characterized. ponA and pbpB encode the penicillin-binding proteins (PBPs) 1 and 3, respectively. There is little sequence variation between the PBP genes from two L. interrogans strains (serovar icterohaemorrhagiae strain Verdun and serovar pomona strain RZ11). The deduced L. interrogans PBP 1 and PBP 3 protein sequences from the two strains shared over 50% similarity to homologous proteins from Escherichia coli. It was demonstrated for strain Verdun that ponA and pbpB are transcribed individually from their own promoter. The ponA and pbpB genes from both strains are separated by 8 to 10 kb and oriented such that their transcription is convergent. The L. interrogans PBP 1 and PBP 3 proteins were synthesized in E. coli and were modified with ampicillin using a digoxigenin-ampicillin conjugate. These data show that both genes encode functional PBPs.

* Corresponding author. Mailing address: Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, 25 rue du docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 (1) 45 68 83 66. Fax: 33 (1) 40 61 30 01. E-mail: isgirons{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, March 2001, p. 870-877, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.870-877.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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