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Antimicrobial Agents and Chemotherapy, March 2001, p. 878-882, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.878-882.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular and Biochemical Analysis of AST-1, a Class A -Lactamase from Nocardia asteroides Sensu Stricto

Laurent Poirel,¹ Frederic Laurent,^1,2,3 Thierry Naas,¹ Roger Labia,⁴ Patrick Boiron,³ and Patrice Nordmann^1,*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex,¹ Service de Bactériologie, Hôpital Lyon-Sud, Hospices Civils de Lyon, Faculté de Médecine Lyon-Sud, 69921 Oullins Cedex,² Laboratoire de mycologie fondamentale et appliquée aux biotechnologies industrielles, Institut des Sciences Pharmaceutiques et Biologiques, 69373 Lyon Cedex 08,³ and UMR175 CNRS Chimie et Biologie des Substances Actives, 29000 Quimper,⁴ France

Received 22 June 2000/Returned for modification 1 October 2000/Accepted 15 December 2000

A -lactamase gene was cloned from a Nocardia asteroides sensu stricto clinical isolate. A recombinant plasmid, pAST-1, expressed the -lactamase AST-1 in Escherichia coli JM109. Its pI was 4.8, and its relative molecular mass was 31 kDa. E. coli JM109(pAST-1) was resistant to penicillins and narrow-spectrum cephalosporins. The -lactamase AST-1 had a restricted hydrolytic activity spectrum. Its activity was partially inhibited by clavulanic acid but not by sulbactam and tazobactam. AST-1 is an Ambler class A -lactamase sharing 65% amino acid identity with -lactamase FAR-1, the most closely related enzyme.

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^* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

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Antimicrobial Agents and Chemotherapy, March 2001, p. 878-882, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.878-882.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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