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Antimicrobial Agents and Chemotherapy, March 2001, p. 973-975, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.973-975.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Restoration of Vancomycin Susceptibility in Enterococcus faecalis by Antiresistance Determinant Gene Transfer

C. Torres Viera,1,2 S. Tsiodras,1,2 H. S. Gold,1,2 E. P. G. Coakley,1,2 C. Wennersten,1 G. M. Eliopoulos,1,2 R. C. Moellering Jr.,1,2 and R. T. Inouye1,2,*

Department of Medicine, Beth Israel Deaconess Medical Center,1 and Harvard Medical School,2 Boston, Massachusetts 02215

Received 13 July 2000/Returned for modification 4 October 2000/Accepted 7 December 2000

We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.

* Corresponding author. Mailing address: Beth Israel Deaconess Medical Center, Division of Infectious Diseases, Kennedy Building, 6th Floor, 1 Deaconess Rd., Boston, MA 02215. Phone: (617) 667-0046. Fax: (617) 975-5235. E-mail: rinouye{at}caregroup.harvard.edu.

Antimicrobial Agents and Chemotherapy, March 2001, p. 973-975, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.973-975.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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