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Antimicrobial Agents and Chemotherapy, April 2001, p. 1109-1114, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1109-1114.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mdt(A), a New Efflux Protein Conferring Multiple Antibiotic Resistance in Lactococcus lactis and Escherichia coli

Vincent Perreten,1,2,3 Franziska V. Schwarz,3 Michael Teuber,3 and Stuart B. Levy1,2,4,*

Center for Adaptation Genetics and Drug Resistance1 and Departments of Molecular Biology and Microbiology2 and of Medicine,4 Tufts University School of Medicine, Boston, Massachusetts 02111, and Laboratory of Food Microbiology, Institute of Food Science, Department of Agriculture and Food Science, Eidgenössische Technische Hochschule Zürich, ETH-Zentrum, CH-8092 Zürich, Switzerland3

Received 8 August 2000/Returned for modification 12 November 2000/Accepted 12 January 2001

The mdt(A) gene, previously designated mef214, from Lactococcus lactis subsp. lactis plasmid pK214 encodes a protein [Mdt(A) (multiple drug transporter)] with 12 putative transmembrane segments (TMS) that contain typical motifs conserved among the efflux proteins of the major facilitator superfamily. However, it also has two C-motifs (conserved in the fifth TMS of the antiporters) and a putative ATP-binding site. Expression of the cloned mdt(A) gene decreased susceptibility to macrolides, lincosamides, streptogramins, and tetracyclines in L. lactis and Escherichia coli, but not in Enterococcus faecalis or in Staphylococcus aureus. Glucose-dependent efflux of erythromycin and tetracycline was demonstrated in L. lactis and in E. coli.

* Corresponding author. Mailing address: Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6764. Fax: (617) 636-0458. E-mail: stuart.levy{at}tufts.edu.

Antimicrobial Agents and Chemotherapy, April 2001, p. 1109-1114, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1109-1114.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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