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Antimicrobial Agents and Chemotherapy, May 2001, p. 1337-1342, Vol. 45, No. 5
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.5.1337-1342.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Synergy of Histone-Derived Peptides of Coho Salmon with Lysozyme and Flounder Pleurocidin

Aleksander Patrzykat,1 Lijuan Zhang,1 Valentina Mendoza,1 George K. Iwama,2 and Robert E. W. Hancock1,*

Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3,1 and Faculty of Agricultural Sciences and AquaNet, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z42

Received 7 August 2000/Returned for modification 18 December 2000/Accepted 29 January 2001

Recent research has identified endogenous cationic antimicrobial peptides as important factors in the innate immunity of many organisms, including fish. It is known that antimicrobial activity, as well as lysozyme activity, can be induced in coho salmon (Oncorhynchus kisutch) mucus after exposure of the fish to infectious agents. Since lysozyme alone does not have antimicrobial activity against Vibrio anguillarum and Aeromonas salmonicida, a four-step protein purification protocol was used to isolate and identify antibacterial fractions from bacterially challenged coho salmon mucus and blood. The purification consisted of extraction with hot acetic acid, extraction and concentration on a C18 cartridge, gel filtration, and reverse-phase chromatography on a C18 column. N-terminal amino acid sequence analyses revealed that both the blood and the mucus antimicrobial fractions demonstrated identity with the N terminus of trout H1 histone. Mass spectroscopic analysis indicated the presence of the entire histone, as well as fragments thereof, including a 26-amino-acid N-terminal segment. These fractions inhibited the growth of antibiotic-supersuscptible Salmonella enterica serovar Typhimurium, as well as A. salmonicida and V. anguillarum. Synthetic peptides identical to the N-terminally acetylated or C-terminally amidated 26-amino-acid fragment were inactive in antimicrobial assays, but they potentiated the antimicrobial activities of the flounder peptide pleurocidin, lysozyme, and crude lysozyme-containing extracts from coho salmon. The peptides bound specifically to anionic lipid monolayers. However, synergy with pleurocidin did not appear to occur at the cell membrane level. The synergistic activities of inducible histone peptides indicate that they play an important role in the first line of salmon defenses against infectious pathogens and that while some histone fragments may have direct antimicrobial effects, others improve existing defenses.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, Vancouver, B.C., Canada V6T 1Z3. Phone: (604) 822-2682. Fax: (604) 822-6041. E-mail: bob{at}cmdr.ubc.ca.

Antimicrobial Agents and Chemotherapy, May 2001, p. 1337-1342, Vol. 45, No. 5
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.5.1337-1342.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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