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Antimicrobial Agents and Chemotherapy, June 2001, p. 1868-1871, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1868-1871.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

CENTA as a Chromogenic Substrate for Studying beta -Lactamases

Carine Bebrone,1 Catherine Moali,1 Florence Mahy,1 Sandrine Rival,2 Jean Denis Docquier,3 Gian Maria Rossolini,3 Jacques Fastrez,4 Rex F. Pratt,5 Jean-Marie Frère,1 and Moreno Galleni1,*

Centre for Protein Engineering, University of Liège, Institut de Chimie B6, Sart Tilman, 4000 Liège,1 and Laboratoire de Biochimie Physique et des Biopolymeres, Université Catholique de Louvain, Bâtiment Lavoisier, 1348 Louvain-la-Neuve,4 Belgium; Fachrichtung 8.8 Biochemie, Universität des Saarlandes, D-66123 Saarbrücken, Germany2; Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena, Italy3; and Department of Chemistry, Wesleyan University, Middletown, Connecticut 064595

Received 23 August 2000/Returned for modification 13 December 2000/Accepted 2 March 2001

CENTA, a chromogenic cephalosporin, is readily hydrolyzed by beta -lactamases of all classes except for the Aeromonas hydrophila metalloenzyme. Although it cannot practically be used for the detection of beta -lactamase-producing strains on agar plates, it should be quite useful for kinetic studies and the detection of the enzymes in crude extracts and chromatographic fractions.

* Corresponding author. Mailing address: Centre for Protein Engineering, Institut de Chimie B6, Sart Tilman, 4000 Liège, Belgium. Phone: 32-4-3663549. Fax: 32-4-3663364. E-mail: mgalleni{at}ulg.ac.be.

Antimicrobial Agents and Chemotherapy, June 2001, p. 1868-1871, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1868-1871.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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