This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alonso, A.
Right arrow Articles by Martinez, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alonso, A.
Right arrow Articles by Martinez, J. L.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2001, p. 1879-1881, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1879-1881.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of Multidrug Efflux Pump SmeDEF by Clinical Isolates of Stenotrophomonas maltophilia

Ana Alonso and Jose L. Martinez*

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain

Received 11 October 2000/Returned for modification 6 February 2000/Accepted 16 March 2001

The presence of the multidrug efflux pump SmeDEF was assessed in a collection of clinical isolates of Stenotrophomonas maltophilia. All isolates encoded this pump, as demonstrated by PCR. Forty-seven percent of the strains overproduced a protein of the same size that was immunoreactive against an anti-SmeF antibody, and 33% overexpressed the gene semD when they were tested by reverse transcription-PCR. A correlation between smeDEF overexpression and antibiotic resistance was observed.

* Corresponding author. Mailing address: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain. Phone: 34-91-5854571. Fax: 34-91-5854506. E-mail: jlmtnez{at}cnb.uam.es.

Antimicrobial Agents and Chemotherapy, June 2001, p. 1879-1881, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1879-1881.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Martinez, J. L. (2009). The role of natural environments in the evolution of resistance traits in pathogenic bacteria. Proc R Soc B 276: 2521-2530 [Abstract] [Full Text]  
  • Hernandez, A., Mate, M. J., Sanchez-Diaz, P. C., Romero, A., Rojo, F., Martinez, J. L. (2009). Structural and Functional Analysis of SmeT, the Repressor of the Stenotrophomonas maltophilia Multidrug Efflux Pump SmeDEF. J. Biol. Chem. 284: 14428-14438 [Abstract] [Full Text]  
  • Falagas, M. E., Valkimadi, P.-E., Huang, Y.-T., Matthaiou, D. K., Hsueh, P.-R. (2008). Therapeutic options for Stenotrophomonas maltophilia infections beyond co-trimoxazole: a systematic review. J Antimicrob Chemother 62: 889-894 [Abstract] [Full Text]  
  • Shimizu, K., Kikuchi, K., Sasaki, T., Takahashi, N., Ohtsuka, M., Ono, Y., Hiramatsu, K. (2008). Smqnr, a New Chromosome-Carried Quinolone Resistance Gene in Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 52: 3823-3825 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Sanchez, P., Moreno, E., Martinez, J. L. (2005). The Biocide Triclosan Selects Stenotrophomonas maltophilia Mutants That Overproduce the SmeDEF Multidrug Efflux Pump. Antimicrob. Agents Chemother. 49: 781-782 [Abstract] [Full Text]  
  • Gould, V. C., Okazaki, A., Howe, R. A., Avison, M. B. (2004). Analysis of sequence variation among smeDEF multi drug efflux pump genes and flanking DNA from defined 16S rRNA subgroups of clinical Stenotrophomonas maltophilia isolates. J Antimicrob Chemother 54: 348-353 [Abstract] [Full Text]  
  • Sanchez, P., Alonso, A., Martinez, J. L. (2004). Regulatory Regions of smeDEF in Stenotrophomonas maltophilia Strains Expressing Different Amounts of the Multidrug Efflux Pump SmeDEF. Antimicrob. Agents Chemother. 48: 2274-2276 [Abstract] [Full Text]  
  • Ba, B. B., Feghali, H., Arpin, C., Saux, M.-C., Quentin, C. (2004). Activities of Ciprofloxacin and Moxifloxacin against Stenotrophomonas maltophilia and Emergence of Resistant Mutants in an In Vitro Pharmacokinetic-Pharmacodynamic Model. Antimicrob. Agents Chemother. 48: 946-953 [Abstract] [Full Text]  
  • Alonso, A., Morales, G., Escalante, R., Campanario, E., Sastre, L., Martinez, J. L. (2004). Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology. J Antimicrob Chemother 53: 432-434 [Abstract] [Full Text]  
  • Chang, L.-L., Chen, H.-F., Chang, C.-Y., Lee, T.-M., Wu, W.-J. (2004). Contribution of integrons, and SmeABC and SmeDEF efflux pumps to multidrug resistance in clinical isolates of Stenotrophomonas maltophilia. J Antimicrob Chemother 53: 518-521 [Abstract] [Full Text]  
  • Huda, N., Lee, E.-W., Chen, J., Morita, Y., Kuroda, T., Mizushima, T., Tsuchiya, T. (2003). Molecular Cloning and Characterization of an ABC Multidrug Efflux Pump, VcaM, in Non-O1 Vibrio cholerae. Antimicrob. Agents Chemother. 47: 2413-2417 [Abstract] [Full Text]  
  • McKay, G. A., Woods, D. E., MacDonald, K. L., Poole, K. (2003). Role of Phosphoglucomutase of Stenotrophomonasmaltophilia in Lipopolysaccharide Biosynthesis, Virulence, and Antibiotic Resistance. Infect. Immun. 71: 3068-3075 [Abstract] [Full Text]  
  • Ruiz, J. (2003). Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. J Antimicrob Chemother 51: 1109-1117 [Abstract] [Full Text]  
  • Sanchez, P., Le, U., Martinez, J. L. (2003). The efflux pump inhibitor Phe-Arg-{beta}-naphthylamide does not abolish the activity of the Stenotrophomonas maltophilia SmeDEF multidrug efflux pump. J Antimicrob Chemother 51: 1042-1045 [Full Text]  
  • Grkovic, S., Brown, M. H., Skurray, R. A. (2002). Regulation of Bacterial Drug Export Systems. Microbiol. Mol. Biol. Rev. 66: 671-701 [Abstract] [Full Text]  
  • Sanchez, P., Alonso, A., Martinez, J. L. (2002). Cloning and Characterization of SmeT, a Repressor of the Stenotrophomonas maltophilia Multidrug Efflux Pump SmeDEF. Antimicrob. Agents Chemother. 46: 3386-3393 [Abstract] [Full Text]  
  • Valdezate, S., Vindel, A., Echeita, A., Baquero, F., Canto, R. (2002). Topoisomerase II and IV Quinolone Resistance-Determining Regions in Stenotrophomonas maltophilia Clinical Isolates with Different Levels of Quinolone Susceptibility. Antimicrob. Agents Chemother. 46: 665-671 [Abstract] [Full Text]  
  • Li, X.-Z., Zhang, L., Poole, K. (2002). SmeC, an Outer Membrane Multidrug Efflux Protein of Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 46: 333-343 [Abstract] [Full Text]  
  • Zhang, L., Li, X.-Z., Poole, K. (2001). SmeDEF Multidrug Efflux Pump Contributes to Intrinsic Multidrug Resistance in Stenotrophomonas maltophilia. Antimicrob. Agents Chemother. 45: 3497-3503 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»