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Antimicrobial Agents and Chemotherapy, June 2001, p. 1934-1936, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1934-1936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method

Ivan Bastian,1,2,* Leen Rigouts,1 Juan Carlos Palomino,1 and Françoise Portaels1

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium,1 and Institute of Medical & Veterinary Science, Adelaide, Australia2

Received 3 November 2000/Returned for modification 18 December 2000/Accepted 16 March 2001

Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 µg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 µg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 µg of kanamycin/ml. Both systems provided results within an average of 1 week.

* Corresponding author. Mailing address: Institute of Medical & Veterinary Science, P.O. Box 14, Rundle Mall, SA 5000, Australia. Phone: 61 8 8222-3000. Fax: 61 8 8222-3543. E-mail: ivan.bastian{at}imvs.sa.gov.au.

Antimicrobial Agents and Chemotherapy, June 2001, p. 1934-1936, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1934-1936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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