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Antimicrobial Agents and Chemotherapy, July 2001, p. 2141-2143, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2141-2143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya

S. Kariuki,1,2,* J. E. Corkill,2 G. Revathi,3 R. Musoke,3 and C. A. Hart2

Centre for Microbiology Research, Kenya Medical Research Institute,1 and Department of Medical Microbiology, Kenyatta National Hospital,3 Nairobi, Kenya, and Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom2

Received 6 December 2000/Returned for modification 20 March 2001/Accepted 21 April 2001

Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum beta -lactamase, which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.

* Corresponding author. Mailing address: Centre for Microbiology Research, Kenya Medical Research Institute, KNH Compound, Off Ngong Rd., P.O. Box 43640, Nairobi, Kenya. Phone: 254-2-720163. Fax: 254-2-711673. E-mail: skariuki{at}wtrl.or.ke.

Antimicrobial Agents and Chemotherapy, July 2001, p. 2141-2143, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2141-2143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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