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Antimicrobial Agents and Chemotherapy, July 2001, p. 2141-2143, Vol. 45, No. 7
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.7.2141-2143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Molecular Characterization of a Novel Plasmid-Encoded
Cefotaximase (CTX-M-12) Found in Clinical Klebsiella
pneumoniae Isolates from Kenya
S.
Kariuki,1,2,*
J. E.
Corkill,2
G.
Revathi,3
R.
Musoke,3 and
C.
A.
Hart2
Centre for Microbiology Research, Kenya
Medical Research Institute,1 and
Department of Medical Microbiology, Kenyatta National
Hospital,3 Nairobi, Kenya, and
Department of Medical Microbiology and Genito-Urinary
Medicine, University of Liverpool, Liverpool L69 3GA, United
Kingdom2
Received 6 December 2000/Returned for modification 20 March
2001/Accepted 21 April 2001
Nine Klebsiella pneumoniae isolates, six from blood
and three from cerebrospinal fluid of newborn babies at Kenyatta
National Hospital, Nairobi, Kenya, were analyzed for the mechanism of
cephalosporin resistance. By using pulsed-field gel electrophoresis of
XbaI-digested chromosomal DNA, all the nine isolates
were found to be clonal. PCR and direct sequencing revealed a novel
extended-spectrum
-lactamase, which we designated CTX-M-12. It has a
more potent hydrolytic activity against cefotaxime than against
ceftazidime and a pI of 9.0 and is encoded on a large self-transferable
ca. 160-kbp plasmid.
*
Corresponding author. Mailing address: Centre for
Microbiology Research, Kenya Medical Research Institute, KNH Compound,
Off Ngong Rd., P.O. Box 43640, Nairobi, Kenya. Phone: 254-2-720163. Fax: 254-2-711673. E-mail: skariuki{at}wtrl.or.ke.
Antimicrobial Agents and Chemotherapy, July 2001, p. 2141-2143, Vol. 45, No. 7
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.7.2141-2143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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