Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya

doi:10.1128/AAC.45.7.2141-2143.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kariuki, S.
	Articles by Hart, C. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kariuki, S.
	Articles by Hart, C. A.

Antimicrobial Agents and Chemotherapy, July 2001, p. 2141-2143, Vol. 45, No. 7
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.7.2141-2143.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of a Novel Plasmid-Encoded Cefotaximase (CTX-M-12) Found in Clinical Klebsiella pneumoniae Isolates from Kenya

S. Kariuki,¹^,²^,^* J. E. Corkill,² G. Revathi,³ R. Musoke,³ and C. A. Hart²

Centre for Microbiology Research, Kenya Medical Research Institute,¹ and Department of Medical Microbiology, Kenyatta National Hospital,³ Nairobi, Kenya, and Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom²

Received 6 December 2000/Returned for modification 20 March 2001/Accepted 21 April 2001

Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta NationalHospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporinresistance. By using pulsed-field gel electrophoresis of XbaI-digestedchromosomal DNA, all the nine isolates were found to be clonal.PCR and direct sequencing revealed a novel extended-spectrum $beta$ -lactamase,which we designated CTX-M-12. It has a more potent hydrolyticactivity against cefotaxime than against ceftazidime and a pIof 9.0 and is encoded on a large self-transferable ca. 160-kbpplasmid.

^* Corresponding author. Mailing address: Centre for Microbiology Research, Kenya Medical Research Institute, KNH Compound, OffNgong Rd., P.O. Box 43640, Nairobi, Kenya. Phone: 254-2-720163.Fax: 254-2-711673. E-mail: skariuki{at}wtrl.or.ke.

This article has been cited by other articles:

Bae, I. K., Lee, Y.-N., Hwang, H. Y., Jeong, S. H., Lee, S. J., Kwak, H.-S., Song, W., Kim, H. J., Youn, H. (2006). Emergence of CTX-M-12 extended-spectrum {beta}-lactamase-producing Escherichia coli in Korea. J Antimicrob Chemother 58: 1257-1259 [Abstract] [Full Text]
Mamlouk, K., Boutiba-Ben Boubaker, I., Gautier, V., Vimont, S., Picard, B., Ben Redjeb, S., Arlet, G. (2006). Emergence and Outbreaks of CTX-M {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Strains in a Tunisian Hospital. J. Clin. Microbiol. 44: 4049-4056 [Abstract] [Full Text]
Valenzuela de Silva, E. M., Mantilla Anaya, J. R., Reguero Reza, M. T., Gonzalez Mejia, E. B., Pulido Manrique, I. Y., Dario Llerena, I., Velandia, D. (2006). Detection of CTX-M-1, CTX-M-15, and CTX-M-2 in Clinical Isolates of Enterobacteriaceae in Bogota, Colombia.. J. Clin. Microbiol. 44: 1919-1920 [Full Text]
Soge, O. O., Queenan, A. M., Ojo, K. K., Adeniyi, B. A., Roberts, M. C. (2006). CTX-M-15 extended-spectrum {beta}-lactamase from Nigerian Klebsiella pneumoniae. J Antimicrob Chemother 57: 24-30 [Abstract] [Full Text]
Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]
Blomberg, B., Jureen, R., Manji, K. P., Tamim, B. S., Mwakagile, D. S. M., Urassa, W. K., Fataki, M., Msangi, V., Tellevik, M. G., Maselle, S. Y., Langeland, N. (2005). High Rate of Fatal Cases of Pediatric Septicemia Caused by Gram-Negative Bacteria with Extended-Spectrum Beta-Lactamases in Dar es Salaam, Tanzania. J. Clin. Microbiol. 43: 745-749 [Abstract] [Full Text]
Fang, H., Lundberg, C., Olsson-Liljequist, B., Hedin, G., Lindback, E., Rosenberg, A., Struwe, J. (2004). Molecular Epidemiological Analysis of Escherichia coli Isolates Producing Extended-Spectrum {beta}-Lactamases for Identification of Nosocomial Outbreaks in Stockholm, Sweden. J. Clin. Microbiol. 42: 5917-5920 [Abstract] [Full Text]
Cartelle, M., del Mar Tomas, M., Molina, F., Moure, R., Villanueva, R., Bou, G. (2004). High-Level Resistance to Ceftazidime Conferred by a Novel Enzyme, CTX-M-32, Derived from CTX-M-1 through a Single Asp240-Gly Substitution. Antimicrob. Agents Chemother. 48: 2308-2313 [Abstract] [Full Text]
Villegas, M. V., Correa, A., Perez, F., Zuluaga, T., Radice, M., Gutkind, G., Casellas, J. M., Ayala, J., Lolans, K., Quinn, J. P., the Colombian Nosocomial Resistance Study Group,, (2004). CTX-M-12 {beta}-Lactamase in a Klebsiella pneumoniae Clinical Isolate in Colombia. Antimicrob. Agents Chemother. 48: 629-631 [Abstract] [Full Text]
Bonnet, R. (2004). Growing Group of Extended-Spectrum {beta}-Lactamases: the CTX-M Enzymes. Antimicrob. Agents Chemother. 48: 1-14 [Full Text]
Nagano, N., Shibata, N., Saitou, Y., Nagano, Y., Arakawa, Y. (2003). Nosocomial Outbreak of Infections by Proteus mirabilis That Produces Extended-Spectrum CTX-M-2 Type {beta}-Lactamase. J. Clin. Microbiol. 41: 5530-5536 [Abstract] [Full Text]
Bonnet, R., Recule, C., Baraduc, R., Chanal, C., Sirot, D., De Champs, C., Sirot, J. (2003). Effect of D240G substitution in a novel ESBL CTX-M-27. J Antimicrob Chemother 52: 29-35 [Abstract] [Full Text]
Neuwirth, C., Siebor, E., Pruneaux, M., Zarnayova, M., Simonin, C., Kisterman, J. P., Labia, R. (2003). First isolation of CTX-M15-producing Escherichia coli from two French patients. J Antimicrob Chemother 51: 471-473 [Full Text]
Poirel, L., Kampfer, P., Nordmann, P. (2002). Chromosome-Encoded Ambler Class A {beta}-Lactamase of Kluyvera georgiana, a Probable Progenitor of a Subgroup of CTX-M Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 46: 4038-4040 [Abstract] [Full Text]
Bou, G., Cartelle, M., Tomas, M., Canle, D., Molina, F., Moure, R., Eiros, J. M., Guerrero, A. (2002). Identification and Broad Dissemination of the CTX-M-14 {beta}-Lactamase in Different Escherichia coli Strains in the Northwest Area of Spain. J. Clin. Microbiol. 40: 4030-4036 [Abstract] [Full Text]
Humeniuk, C., Arlet, G., Gautier, V., Grimont, P., Labia, R., Philippon, A. (2002). {beta}-Lactamases of Kluyvera ascorbata, Probable Progenitors of Some Plasmid-Encoded CTX-M Types. Antimicrob. Agents Chemother. 46: 3045-3049 [Abstract] [Full Text]
Ma, L., Ishii, Y., Chang, F.-Y., Yamaguchi, K., Ho, M., Siu, L. K. (2002). CTX-M-14, a Plasmid-Mediated CTX-M Type Extended-Spectrum {beta}-Lactamase Isolated from Escherichia coli. Antimicrob. Agents Chemother. 46: 1985-1988 [Abstract] [Full Text]
Cao, V., Lambert, T., Courvalin, P. (2002). ColE1-Like Plasmid pIP843 of Klebsiella pneumoniae Encoding Extended-Spectrum {beta}-Lactamase CTX-M-17. Antimicrob. Agents Chemother. 46: 1212-1217 [Abstract] [Full Text]
Petroni, A., Corso, A., Melano, R., Cacace, M. L., Bru, A. M., Rossi, A., Galas, M. (2002). Plasmidic Extended-Spectrum {beta}-Lactamases in Vibrio cholerae O1 El Tor Isolates in Argentina. Antimicrob. Agents Chemother. 46: 1462-1468 [Abstract] [Full Text]
Chanawong, A., M'Zali, F. H., Heritage, J., Xiong, J.-H., Hawkey, P. M. (2002). Three Cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China. Antimicrob. Agents Chemother. 46: 630-637 [Abstract] [Full Text]
Coque, T. M., Oliver, A., Perez-Diaz, J. C., Baquero, F., Canton, R. (2002). Genes Encoding TEM-4, SHV-2, and CTX-M-10 Extended-Spectrum {beta}-Lactamases Are Carried by Multiple Klebsiella pneumoniae Clones in a Single Hospital (Madrid, 1989 to 2000). Antimicrob. Agents Chemother. 46: 500-510 [Abstract] [Full Text]
Baraniak, A., Fiett, J., Sulikowska, A., Hryniewicz, W., Gniadkowski, M. (2002). Countrywide Spread of CTX-M-3 Extended-Spectrum {beta}-Lactamase-Producing Microorganisms of the Family Enterobacteriaceae in Poland. Antimicrob. Agents Chemother. 46: 151-159 [Abstract] [Full Text]
Poirel, L., Naas, T., Le Thomas, I., Karim, A., Bingen, E., Nordmann, P. (2001). CTX-M-Type Extended-Spectrum beta -Lactamase That Hydrolyzes Ceftazidime through a Single Amino Acid Substitution in the Omega Loop. Antimicrob. Agents Chemother. 45: 3355-3361 [Abstract] [Full Text]
Decousser, J. W., Poirel, L., Nordmann, P. (2001). Characterization of a Chromosomally Encoded Extended-Spectrum Class A beta -Lactamase from Kluyvera cryocrescens. Antimicrob. Agents Chemother. 45: 3595-3598 [Abstract] [Full Text]

Clin. Vaccine Immunol.	Clin. Microbiol. Rev.
J. Clin. Microbiol.	ALL ASM JOURNALS