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Antimicrobial Agents and Chemotherapy, August 2001, p. 2224-2228, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2224-2228.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Metallo-beta -Lactamases in Clinical Pseudomonas Isolates in Taiwan and Identification of VIM-3, a Novel Variant of the VIM-2 Enzyme

Jing-Jou Yan,1 Po-Ren Hsueh,2 Wen-Chien Ko,3 Kwen-Tay Luh,2 Shu-Huei Tsai,1 Hsiu-Mei Wu,4 and Jiunn-Jong Wu4,*

Department of Pathology, National Cheng Kung University Medical Center,1 and Departments of Medicine3 and Medical Technology,4 National Cheng Kung University Medical College, Tainan, Department of Laboratory Medicine, National Taiwan University Hospital, Taipei,2 Taiwan

Received 9 October 2000/Returned for modification 29 January 2001/Accepted 4 May 2001

A total of 209 clinical isolates of Pseudomonas (193 Pseudomonas aeruginosa, 10 P. putida, 4 P. stutzeri, and 2 P. fluorescens isolates) with reduced susceptibilities to imipenem and/or ceftazidime were subjected to PCR assays with primers specific for blaIMP-1, blaIMP-2, blaVIM-1, and blaVIM-2 and sequence analysis to identify the metallo-beta -lactamases (MBLs) prevalent among these organisms in Taiwan; and 21 isolates gave positive results. Five isolates including two P. putida and three P. stutzeri isolates were found to carry blaIMP-1, and six isolates including five P. putida and one P. stutzeri isolates harbored blaVIM-2. The remaining 10 isolates were P. aeruginosa, and all were found to carry a novel variant of blaVIM-2, designated blaVIM-3. There are only two nucleotide differences between blaVIM-2 and blaVIM-3, leading to two amino acid alterations. Our findings indicate that VIM-2 and its variant have become the most prevalent metalloenzymes in Pseudomonas in Taiwan. Southern hybridization with the blaVIM-2-, blaVIM-3-, and blaIMP-1 -specific probes revealed that only two VIM-2-producing P. putida isolates appeared to carry the MBL gene on plasmids. Pulsed-field gel electrophoresis showed that six VIM-3-producing P. aeruginosa isolates and two IMP-1-producing P. stutzeri isolates were genetically related, suggesting that the spread of these MBL genes in Taiwan could be due to clonal dissemination as well as genetic exchange between different clones.

* Corresponding author. Mailing address: Department of Medical Technology, National Cheng Kung University Medical College, No. 1 University Rd., Tainan, Taiwan 70101. Phone: 886-6-2353535, ext. 5775. Fax: 886-6-2363956. E-mail: jjwu{at}mail.ncku.edu.tw.

Antimicrobial Agents and Chemotherapy, August 2001, p. 2224-2228, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2224-2228.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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