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Antimicrobial Agents and Chemotherapy, August 2001, p. 2324-2330, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2324-2330.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Characterization of Chromosomal Class C beta -Lactamase and Its Regulatory Gene in Ochrobactrum anthropi

David Nadjar,1 Roger Labia,2 Claude Cerceau,2 Chantal Bizet,3 Alain Philippon,4 and Guillaume Arlet1,*

Laboratoire de Bactériologie, Hôpital Tenon, UFR Saint-Antoine,1 Collection de l'Institut Pasteur, Institut Pasteur,3 and Service de Bactériologie, CHU Cochin,4 Paris, and CNRS-UBO-MHN, Unité FRE 2125, Quimper,2 France

Received 28 December 2000/Returned for modification 9 April 2001/Accepted 26 May 2001

Ochrobactrum anthropi, formerly known as CDC group Vd, is an oxidase-producing, gram-negative, obligately aerobic, non-lactose-fermenting bacillus of low virulence that occasionally causes human infections. It is highly resistant to all beta -lactams except imipenem. A clinical isolate, SLO74, and six reference strains were tested. MICs of penicillins, aztreonam, and most cephalosporins tested, including cefotaxime and ceftazidime, were >128 µg/ml and of cefepime were 64 to >128 µg/ml. Clavulanic acid was ineffective and tazobactam had a weak effect in association with piperacillin. Two genes, ampR and ampC, were cloned by inserting restriction fragments of genomic DNA from the clinical strain O. anthropi SLO74 into pBK-CMV to give the recombinant plasmid pBK-OA1. The pattern of resistance to beta -lactams of this clone was similar to that of the parental strain, except for its resistance to cefepime (MIC, 0.5 µg/ml). The deduced amino acid sequence of the AmpC beta -lactamase (pI, 8.9) was only 41 to 52% identical to the sequence of other chromosomally encoded and plasmid-encoded class C beta -lactamases. The kinetic properties of this beta -lactamase were typical for this class of beta -lactamases. Upstream from the ampC gene, the ampR gene encodes a protein with a sequence that is 46 to 62% identical to those of other AmpR proteins and with an amino-terminal DNA-binding domain typical of transcriptional activators of the Lys-R family. The deduced amino acid sequences of the ampC genes of the six reference strains were 96 to 99% identical to the sequence of the clinical strain. The beta -lactamase characterized from strain SLO74 was named OCH-1 (gene, blaOCH-I).

* Corresponding author. Mailing address: Service de Bactériologie, Hôpital Tenon, 4, rue de la Chine, 75970 Paris Cedex 20, France. Phone: 33 1 56 01 70 18. Fax: 33 1 56 01 61 08. E-mail: guillaume.arlet{at}tnn.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, August 2001, p. 2324-2330, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2324-2330.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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