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Antimicrobial Agents and Chemotherapy, August 2001, p. 2378-2380, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2378-2380.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mutation in the DNA Gyrase A Gene of Escherichia coli That Expands the Quinolone Resistance-Determining Region

S. Marvin Friedman,1,* Tao Lu,2 and Karl Drlica2

Department of Biological Sciences, Hunter College of The City University of New York, New York, New York 10021,1 and Public Health Research Institute, New York, New York 100162

Received 25 January 2001/Returned for modification 13 March 2001/Accepted 2 May 2001

In three Escherichia coli mutants, a change (Ala-51 to Val) in the gyrase A protein outside the standard quinolone resistance-determining region (QRDR) lowered the level of quinolone susceptibility more than changes at amino acids 67, 82, 84, and 106 did. Revision of the QRDR to include amino acid 51 is indicated.

* Corresponding author. Mailing Address: Department of Biological Sciences, Hunter College of The City University of New York, 695 Park Ave., New York, N.Y. 10021. Phone: (212) 772-5608. Fax: (212) 772-5227. E-mail: friedman{at}genectr.hunter.cuny.edu.

Antimicrobial Agents and Chemotherapy, August 2001, p. 2378-2380, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2378-2380.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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