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Antimicrobial Agents and Chemotherapy, August 2001, p. 2386-2389, Vol. 45, No. 8
Laboratoire de Pathobiologie Orale,
Faculté de Chirurgie Dentaire, 06357 Nice Cedex
4,1 Laboratoire de Bactériologie,
Hôpital l'Archet 2, 06202 Nice Cedex 3,2
Laboratoire de Biochimie, INSERM 145, Faculté de
Médecine, 06107 Nice Cedex 2,3 and
CNRS, UMR 175, 29000 Quimper,4 France
Received 1 August 2000/Returned for modification 10 November
2000/Accepted 11 May 2001
The gene encoding a
0066-4804/01/$04.00+0 DOI: 10.1128/AAC.45.8.2386-2389.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Cloning and Biochemical Characterization of a Class A
-Lactamase from Prevotella intermedia
-lactamase of Prevotella
intermedia was cloned and sequenced. This gene, called
cfxA2, shared 98% identity with cfxA, the
structural gene of a
-lactamase previously described in
Bacteroides vulgatus. The deduced protein sequence had a
K272E substitution. CfxA2 had the characteristics of class A, group 2e
-lactamases.
*
Corresponding author. Mailing address: Laboratoire de
Bactériologie, Hôpital l'Archet 2, 151, route de Saint
Antoine Ginestière, 06202 Nice Cedex 3, France. Phone: (33) (4)
92 03 62 14. Fax: (33) (4) 90 03 65 49. E-mail:
fosse{at}unice.fr.
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