This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kano, R.
Right arrow Articles by Hasegawa, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kano, R.
Right arrow Articles by Hasegawa, A.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, September 2001, p. 2559-2562, Vol. 45, No. 9
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.9.2559-2562.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of Ubiquitin Gene in Microsporum canis and Trichophyton mentagrophytes Cultured with Fluconazole

Rui Kano,1,* Ken Okabayashi,1 Yuka Nakamura,2 Shinichi Watanabe,2 and Atsuhiko Hasegawa1

Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa Kanagawa 252-8510,1 and Department of Dermatology, Teikyo University School of Medicine, Itabashi-ku, Tokyo 173-8605,2 Japan

Received 21 July 2000/Returned for modification 15 February 2001/Accepted 18 June 2001

The expression of the ubiquitin (Ub) gene in dermatophytes was examined for its relation to resistance against the antifungal drug fluconazole. The nucleotide sequences and the deduced amino acid sequences of the Ub gene in Microsporum canis were proven to be 99% similar to those of the Ub gene in Trichophyton mentagrophytes. Expression of mRNA of Ub in M. canis and T. mentagrophytes was enhanced when the fungi were cultured with fluconazole. The antifungal activity of fluconazole against these dermatophytes was increased in the presence of Ub proteasome inhibitor.

* Corresponding author. Mailing address: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa Kanagawa 252-8510, Japan. Phone: 81-466-84-3649. Fax: 81-466-84-3649. E-mail: kano{at}brs.nihon-u.ac.jp.

Antimicrobial Agents and Chemotherapy, September 2001, p. 2559-2562, Vol. 45, No. 9
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.9.2559-2562.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»