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Antimicrobial Agents and Chemotherapy, October 2002, p. 3215-3222, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3215-3222.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biochemical-Genetic Analysis and Distribution of DES-1, an Ambler Class A Extended-Spectrum ß-Lactamase from Desulfovibrio desulfuricans

Anne-Sophie Morin,1 Laurent Poirel,1 Francine Mory,2 Roger Labia,3 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre,1 Laboratoire de Bactériologie, Hôpital Central, 54035 Nancy Cedex,2 UMR175 CNRS Chimie et Biologie des Substances Actives, 29000 Quimper, France3

Received 8 November 2001/ Returned for modification 9 March 2002/ Accepted 8 July 2002

Desulfovibrio spp. are gram-negative anaerobes phylogenetically related to Bacteroides spp., which are rarely isolated and which are mostly isolated from intra-abdominal abscesses. Desulfovibrio desulfuricans clinical isolate D3 had a clavulanic acid-inhibited ß-lactam resistance profile and was resistant to some expanded-spectrum cephalosporins. A ß-lactamase gene, blaDES-1, was cloned from whole-cell DNA of isolate D3 and expressed in Escherichia coli. Purified ß-lactamase DES-1, with a pI value of 9.1, had a relative molecular mass of ca. 31 kDa and a mature protein of 288 amino acids. DES-1 was distantly related to Ambler class A ß-lactamases and most closely related to PenA from Burkholderia pseudomallei (48% amino acid identity). It was weakly related to class A ß-lactamases CblA, CepA, CfxA, and CfxA2 from other anaerobic species, Bacteroides spp. and Prevotella intermedia. Its hydrolysis spectrum included amino- and ureidopenicillins, narrow-spectrum cephalosporins, ceftriaxone, and cefoperazone. blaDES-1-like genes were not identified in phylogenetically related Desulfovibrio fairfieldensis isolates. However, they were found in some but not all D. desulfuricans strains, thus suggesting that these genes may be present in a given D. desulfuricans subspecies.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, October 2002, p. 3215-3222, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3215-3222.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  


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