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Antimicrobial Agents and Chemotherapy, October 2002, p. 3253-3256, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3253-3256.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations in the aph(2")-Ic Gene Are Responsible for Increased Levels of Aminoglycoside Resistance

Hae Kyung Lee,1 Sergei B. Vakulenko,1,2 Don B. Clewell,3,4 Stephen A. Lerner,1,2 and Joseph W. Chow1,3,5*

Division of Infectious Diseases,1 Department of Biochemistry and Molecular Biology, Wayne State University,2 Research and Medical Services, John D. Dingell Veterans Affairs Medical Center, Detroit, Michigan 48201,5 Departments of Biologic and Materials Sciences,3 Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan 481094

Received 28 January 2002/ Returned for modification 21 April 2002/ Accepted 24 June 2002

Random PCR mutagenesis of the enterococcal aph(2")-Ic gene followed by selection for mutant enzymes that confer enhanced levels of aminoglycoside resistance resulted in mutants of APH(2")-Ic with His-258-Leu and Phe-108-Leu substitutions, all of which conferred rises in the MICs of several aminoglycosides. The mutated residues are located outside conserved regions of aminoglycoside phosphotransferases.

* Corresponding author. Mailing address: Medical Service, John D. Dingell Veterans Affairs Medical Center, 4646 John R, Detroit, MI 48201-1932. Phone: (313) 576-3310. Fax: (313) 993-0302. E-mail: JChow{at}wayne.edu.

Antimicrobial Agents and Chemotherapy, October 2002, p. 3253-3256, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3253-3256.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Badarau, A., Shi, Q., Chow, J. W., Zajicek, J., Mobashery, S., Vakulenko, S. (2008). Aminoglycoside 2''-Phosphotransferase Type IIIa from Enterococcus. J. Biol. Chem. 283: 7638-7647 [Abstract] [Full Text]  


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