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Antimicrobial Agents and Chemotherapy, October 2002, p. 3261-3264, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3261-3264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Penicillin-Binding Protein 1A, 2B, and 2X Alterations in Canadian Isolates of Penicillin-Resistant Streptococcus pneumoniae

Kimberly A. Nichol,1,2* George G. Zhanel,1,2,3,4 and Daryl J. Hoban1,2

Department of Medical Microbiology, Faculty of Medicine,1 Faculty of Pharmacy, University of Manitoba,3 Departments of Medicine,4 Clinical Microbiology, Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada2

Received 30 October 2001/ Returned for modification 16 March 2002/ Accepted 10 July 2002

Alterations within the penicillin-binding domain of penicillin-binding protein (PBP) genes pbp1a, pbp2b, and pbp2x were determined for 15 Canadian isolates of Streptococcus pneumoniae. All penicillin-nonsusceptible S. pneumoniae isolates showed a variety of PBP 2X substitutions and contained a Thr445-Ala change after the PBP 2B SSN motif. Only isolates for which penicillin MICs were >=0.5 µg/ml had PBP 1A alterations near the STMK and SRN motifs. Sequence analysis revealed identical PBP 1A, PBP 2B, and PBP 2X substitution patterns among all isolates for which penicillin MICs were >=1 µg/ml.

* Corresponding author. Mailing address: Clinical Microbiology, Health Sciences Centre, MS673-820 Sherbrook St., Winnipeg, Manitoba R3A 1R9, Canada. Phone: (204) 787-4684. Fax: (204) 787-4699. E-mail: knichol{at}hsc.mb.ca.

Antimicrobial Agents and Chemotherapy, October 2002, p. 3261-3264, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3261-3264.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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