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Antimicrobial Agents and Chemotherapy, November 2002, p. 3422-3427, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3422-3427.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Complete Nucleotide Sequence of Klebsiella pneumoniae Multiresistance Plasmid pJHCMW1

Renee Sarno,1 Glen McGillivary,2 David J. Sherratt,3 Luis A. Actis,2 and Marcelo E. Tolmasky1,3*

Department of Biological Science, Institute of Molecular Biology and Nutrition, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, California 92834-6850,1 Department of Microbiology, Miami University, Oxford, Ohio 45056,2 Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom3

Received 5 March 2002/ Returned for modification 12 June 2002/ Accepted 16 August 2002

The multiresistance plasmid pJHCMW1, harbored by a clinical Klebsiella pneumoniae strain isolated from a neonate with meningitis, was sequenced. A circular sequence of 11,354 bp was generated, of which 7,993 bp make up Tn1331, a transposon including the antibiotic resistance genes aac(6')-Ib, aadA1, blaOXA-9, and blaTEM-1. The gene aac(6')-Ib is included in a gene cassette, and both aadA1 and blaOXA-9 are included in a single-gene cassette that may have arisen as a consequence of a recombination event involving two integrons. The pJHCMW1 plasmid replicates through a ColE1-like RNA-regulated mechanism, includes a functional oriT, and two loci with similarity to XerCD site-specific recombination target sites involved in plasmid stabilization by the resolution of multimers. One of these two loci, mwr, is active and has been the subject of previous studies, and the other, dxs, is not functional but binds the recombinase XerD with low affinity. Two additional open reading frames were identified, one with low similarity to two hypothetical membrane proteins from Mycobacterium tuberculosis and Mycobacterium leprae and the other with low similarity to psiB, a gene encoding a function that facilitates the establishment of the transferring plasmid in the recipient bacterial cell during the process of conjugation.

* Corresponding author. Mailing address: Department of Biological Science, Institute of Molecular Biology and Nutrition, College of Natural Science and Mathematics, California State University Fullerton, Fullerton, CA 92834-6850. Phone: (714) 278-5263. Fax: (714) 278-3426. E-mail: mtolmasky{at}fullerton.edu.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3422-3427, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3422-3427.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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