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Antimicrobial Agents and Chemotherapy, November 2002, p. 3591-3596, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3591-3596.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Vitro Activity of Caspofungin against Candida albicans Biofilms

Stefano P. Bachmann,1 Kacy VandeWalle,1 Gordon Ramage,2 Thomas F. Patterson,1 Brian L. Wickes,2 John R. Graybill,1 and José L. López-Ribot1,2*

Department of Medicine, Division of Infectious Diseases,1 Department of Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas2

Received 5 April 2002/ Returned for modification 15 May 2002/ Accepted 23 July 2002

Most manifestations of candidiasis are associated with biofilm formation on biological or inanimate surfaces. Candida albicans biofilms are recalcitrant to treatment with conventional antifungal therapies. Here we report on the activity of caspofungin, a new semisynthetic echinocandin, against C. albicans biofilms. Caspofungin displayed potent in vitro activity against sessile C. albicans cells within biofilms, with MICs at which 50% of the sessile cells were inhibited well within the drug's therapeutic range. Scanning electron microscopy and confocal scanning laser microscopy were used to visualize the effects of caspofungin on preformed C. albicans biofilms, and the results indicated that caspofungin affected the cellular morphology and the metabolic status of cells within the biofilms. The coating of biomaterials with caspofungin had an inhibitory effect on subsequent biofilm development by C. albicans. Together these findings indicate that caspofungin displays potent activity against C. albicans biofilms in vitro and merits further investigation for the treatment of biofilm-associated infections.

* Corresponding author. Mailing address: Department of Medicine/Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, South Texas Centers for Biology in Medicine, Texas Research Park, 15355 Lambda Dr., San Antonio, TX 78245. Phone: (210) 562-5017. Fax: (210) 562-5016. E-mail: RIBOT{at}UTHSCSA.EDU.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3591-3596, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3591-3596.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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