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Antimicrobial Agents and Chemotherapy, November 2002, p. 3634-3636, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3634-3636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Vitro Pharmacodynamic Properties of Three Antifungal Agents against Preformed Candida albicans Biofilms Determined by Time-Kill Studies

Gordon Ramage,1 Kacy VandeWalle,2 Stefano P. Bachmann,2 Brian L. Wickes,1 and José L. López-Ribot1,2*

Department of Microbiology,1 Division of Infectious Diseases, Department of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas2

Received 6 May 2002/ Returned for modification 10 June 2002/ Accepted 13 August 2002

We have examined the in vitro activities of fluconazole, amphotericin B, and caspofungin against Candida albicans biofilms by time-kill methodology. Fluconazole was ineffective against biofilms. Killing of biofilm cells was suboptimal at therapeutic concentrations of amphotericin B. Caspofungin displayed the most effective pharmacokinetic properties, with >=99% killing at physiological concentrations.

* Corresponding author. Mailing address: Department of Medicine/Division of Infectious Diseases, The University of Texas Health Science Center at San Antonio, South Texas Centers for Biology in Medicine, Texas Research Park, 15355 Lambda Dr., San Antonio, TX 78245. Phone: (210) 562-5017. Fax: (210) 562-5016. E-mail: RIBOT{at}UTHSCSA.EDU.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3634-3636, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3634-3636.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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