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Antimicrobial Agents and Chemotherapy, November 2002, p. 3644-3647, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3644-3647.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Comparative Evaluation of NCCLS M27-A and EUCAST Broth Microdilution Procedures for Antifungal Susceptibility Testing of Candida Species

Manuel Cuenca-Estrella,1 Wendy Lee-Yang,2 Meral A. Ciblak,2 Beth A. Arthington-Skaggs,2 Emilia Mellado,1 David W. Warnock,2 and Juan L. Rodriguez-Tudela1*

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain,1 Mycotic Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 23 May 2002/ Returned for modification 28 June 2002/ Accepted 14 August 2002

A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the agreement was 92% and the intraclass correlation coefficient was 0.90 (P < 0.05).

* Corresponding author. Mailing address: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. de Majadahonda a Pozuelo Km. 2, 28220 Majadahonda, Spain. Phone: 34 91 5097961. Fax: 34 91 5097966. E-mail: juanl.rodriguez-tudela{at}isciii.es.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3644-3647, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3644-3647.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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