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Antimicrobial Agents and Chemotherapy, November 2002, p. 3679-3682, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3679-3682.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of SHV-2 ß-Lactamase and of Reduced Amounts of OmpK36 Porin in Klebsiella pneumoniae Results in Increased Resistance to Cephalosporins and Carbapenems

Brendan Crowley,1 Vicente J. Benedí,2* and Antonio Doménech-Sánchez2

Public Health Laboratory, University Hospital Aintree, Liverpool L9 7AL, United Kingdom,1 Laboratorio de Microbiología, Instituto Mediterráneo de Estudios Avanzados (IMEDEA, CSIC-UIB), Universitat de les Illes Balears, 07071-Palma de Mallorca, Spain2

Received 13 February 2002/ Returned for modification 22 May 2002/ Accepted 9 August 2002

A Klebsiella pneumoniae clinical isolate was resistant to cefoxitin, cefotaxime, ceftazidime, ceftazidime-clavulanate, piperacillin-tazobactam (MICs, >256 µg/ml in all cases), and meropenem (MIC, 16 µg/ml) and was intermediate to imipenem (MIC, 8 µg/ml). Decreased expression of the OmpK36 porin and expression of an SHV-2 ß-lactamase contributed to the observed resistance to these ß-lactam-containing agents.

* Corresponding author. Mailing address: Laboratorio de Microbiología, Instituto Mediterráneo de Estudios Avanzados (IMEDEA, CSIC-UIB), Carretera de Valldemosa Km. 7,5, 07071-Palma de Mallorca, Spain. Phone: 34-971-173353. Fax: 34-971-173184. E-mail: vjbenedi{at}uib.es.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3679-3682, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3679-3682.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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