This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dromer, F. Articles by Poulain, D. Search for Related Content PubMed PubMed Citation Articles by Dromer, F. Articles by Poulain, D.

Synthetic Analogues of ß-1,2 Oligomannosides Prevent Intestinal Colonization by the Pathogenic Yeast Candida albicans

Unité de Mycologie Moléculaire, Institut Pasteur, 75015 Paris,¹ Département de Chimie, Ecole Normale Supérieure, 75005 Paris,² Equipe Inserm 9915, Faculté de Médecine, Pôle Recherche, Centre Hospitalier-Universitaire, 59045 Lille,³ Laboratoire de Parasitologie, Faculté de Pharmacie, Boulevard Daviers, 49000 Angers, France⁴

Received 1 April 2002/ Returned for modification 13 May 2002/ Accepted 15 July 2002

The pathogenic yeast Candida albicans displays at its cell surface ß-1,2 oligomannosides (ß-1,2-Mans). In contrast to the ubiquitous -Mans, ß-1,2-Mans bind to galectin-3, a major endogenous lectin expressed on epithelial cells. The specific role of ß-1,2-Mans in colonization of the gut by C. albicans was assessed in a mouse model. A selected virulent strain of C. albicans (expressing more ß-1,2-Man epitopes) induced more intense and sustained colonization than an avirulent strain (expressing less ß-1,2-Man epitopes). Synthetic () ß-and -linked tetramannosides with antigenicities that mimicked the antigenicities of C. albicans-derived oligomannosides were then constructed. Oral administration of ß-1,2-Man (30 mg/kg of body weight) prior to inoculation with the virulent strain resulted in almost complete eradication of yeasts from stool samples, whereas administration of -Man at the same dose did not. As most cases of human systemic candidiasis are endogenous in origin, this first demonstration that a synthetic analogue of a yeast adhesin can prevent yeast colonization in the gut opens the possibility of new prophylactic strategies.

This article has been cited by other articles:

• Mille, C., Bobrowicz, P., Trinel, P.-A., Li, H., Maes, E., Guerardel, Y., Fradin, C., Martinez-Esparza, M., Davidson, R. C., Janbon, G., Poulain, D., Wildt, S. (2008). Identification of a New Family of Genes Involved in {beta}-1,2-Mannosylation of Glycans in Pichia pastoris and Candida albicans. J. Biol. Chem. 283: 9724-9736 [Abstract] [Full Text]  
• Satoh, T., Cowieson, N. P., Hakamata, W., Ideo, H., Fukushima, K., Kurihara, M., Kato, R., Yamashita, K., Wakatsuki, S. (2007). Structural Basis for Recognition of High Mannose Type Glycoproteins by Mammalian Transport Lectin VIP36. J. Biol. Chem. 282: 28246-28255 [Abstract] [Full Text]  
• Kohatsu, L., Hsu, D. K., Jegalian, A. G., Liu, F.-T., Baum, L. G. (2006). Galectin-3 Induces Death of Candida Species Expressing Specific beta-1,2-Linked Mannans. J. Immunol. 177: 4718-4726 [Abstract] [Full Text]  
• Mille, C., Janbon, G., Delplace, F., Ibata-Ombetta, S., Gaillardin, C., Strecker, G., Jouault, T., Trinel, P.-A., Poulain, D. (2004). Inactivation of CaMIT1 Inhibits Candida albicans Phospholipomannan {beta}-Mannosylation, Reduces Virulence, and Alters Cell Wall Protein {beta}-Mannosylation. J. Biol. Chem. 279: 47952-47960 [Abstract] [Full Text]  
• Masuoka, J. (2004). Surface Glycans of Candida albicans and Other Pathogenic Fungi: Physiological Roles, Clinical Uses, and Experimental Challenges. Clin. Microbiol. Rev. 17: 281-310 [Abstract] [Full Text]  
• Dalle, F., Jouault, T., Trinel, P. A., Esnault, J., Mallet, J. M., d'Athis, P., Poulain, D., Bonnin, A. (2003). {beta}-1,2- and {alpha}-1,2-Linked Oligomannosides Mediate Adherence of Candida albicans Blastospores to Human Enterocytes In Vitro. Infect. Immun. 71: 7061-7068 [Abstract] [Full Text]