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Antimicrobial Agents and Chemotherapy, December 2002, p. 3984-3986, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3984-3986.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

AcrAB Efflux System: Expression and Contribution to Fluoroquinolone Resistance in Klebsiella spp.

Annarita Mazzariol,1* Jessica Zuliani,1 Giuseppe Cornaglia,1 Gian Maria Rossolini,2 and Roberta Fontana1

Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona, 37134 Verona,1 Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 Siena, Italy2

Received 1 April 2002/ Returned for modification 15 May 2002/ Accepted 5 September 2002

Seven Klebsiella pneumoniae and four Klebsiella oxytoca clinical isolates with different levels of resistance to ciprofloxacin were studied. Mutations in the topoisomerase genes were found in almost all strains, but the contribution of a multidrug efflux system homologous to AcrAB in Escherichia coli was also observed. Overexpression of this efflux system was demonstrated by immunoblotting with antibodies against E. coli AcrA.

* Corresponding author. Mailing address: Dipartimento di Patologia, Sezione di Microbiologia, Strada Le Grazie, 8, 37134 Verona Italy. Phone: 0039-045-8027690. Fax: 0039-045-584606. E-mail: annarita.mazzariol{at}univr.it.

Antimicrobial Agents and Chemotherapy, December 2002, p. 3984-3986, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3984-3986.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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