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Antimicrobial Agents and Chemotherapy, February 2002, p. 315-320, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.315-320.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Bacilysocin, a Novel Phospholipid Antibiotic Produced by Bacillus subtilis 168

Norimasa Tamehiro,1 Yoshiko Okamoto-Hosoya,1 Susumu Okamoto,1 Makoto Ubukata,2 Masa Hamada,3 Hiroshi Naganawa,3 and Kozo Ochi1*

National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan,1 Toyama Prefectural University, Kosugi, Toyama 939-0398, Japan,2 Institute of Microbial Chemistry, Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan3

Received 1 August 2001/ Returned for modification 7 September 2001/ Accepted 5 November 2001

We have found a novel phospholipid antibiotic (named bacilysocin) which accumulates within (or associates with) the cells of Bacillus subtilis 168 and determined the structure by nuclear magnetic resonance and mass spectrometry analyses. The structure of bacilysocin elucidated was 1-(12-methyltetradecanoyl)-3-phosphoglyceroglycerol. Bacilysocin demonstrated antimicrobial activity, especially against certain fungi. Production of bacilysocin commenced immediately after growth ceased and before the formation of heat-resistant spores. The production of bacilysocin was completely blocked when the ytpA gene, which encodes a protein homologous to lysophospholipase, was disrupted, but blockage of the ytpA gene did not significantly affect growth. Sporulation was also impaired, with a 10-fold reduction in heat-resistant spore titers being detected. Since the ytpA disruptant actually lacked phospholipase activity, we propose that the YtpA protein functions as an enzyme for the biosynthesis of bacilysocin.

* Corresponding author. Mailing address: National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan. Phone: 81-298-38-8125. Fax: 81-298-38-7996. E-mail: kochi{at}affrc.go.jp.

Antimicrobial Agents and Chemotherapy, February 2002, p. 315-320, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.315-320.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Strauch, M. A., Bobay, B. G., Cavanagh, J., Yao, F., Wilson, A., Le Breton, Y. (2007). Abh and AbrB Control of Bacillus subtilis Antimicrobial Gene Expression. J. Bacteriol. 189: 7720-7732 [Abstract] [Full Text]  
  • Inaoka, T., Takahashi, K., Ohnishi-Kameyama, M., Yoshida, M., Ochi, K. (2003). Guanine Nucleotides Guanosine 5'-Diphosphate 3'-Diphosphate and GTP Co-operatively Regulate the Production of an Antibiotic Bacilysin in Bacillus subtilis. J. Biol. Chem. 278: 2169-2176 [Abstract] [Full Text]  


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