This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goñi-Urriza, M.
Right arrow Articles by Quentin, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goñi-Urriza, M.
Right arrow Articles by Quentin, C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2002, p. 350-359, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.350-359.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Type II Topoisomerase Quinolone Resistance-Determining Regions of Aeromonas caviae, A. hydrophila, and A. sobria Complexes and Mutations Associated with Quinolone Resistance

Marisol Goñi-Urriza,1,2 Corinne Arpin,1 Michèle Capdepuy,1 Véronique Dubois,1 Pierre Caumette,2 and Claudine Quentin1*

Laboratoire de Microbiologie, Université de Bordeaux 2, Bordeaux,1 Laboratoire d'Ecologie Moléculaire, Université de Pau, Pau, France2

Received 16 March 2001/ Returned for modification 20 April 2001/ Accepted 2 November 2001

Most Aeromonas strains isolated from two European rivers were previously found to be resistant to nalidixic acid. In order to elucidate the mechanism of this resistance, 20 strains of Aeromonas caviae (n = 10), A. hydrophila (n = 5), and A. sobria (n = 5) complexes, including 3 reference strains and 17 environmental isolates, were investigated. Fragments of the gyrA, gyrB, parC, and parE genes encompassing the quinolone resistance-determining regions (QRDRs) were amplified by PCR and sequenced. Results obtained for the six sensitive strains showed that the GyrA, GyrB, ParC, and ParE QRDR fragments of Aeromonas spp. were highly conserved (>=96.1% identity), despite some genetic polymorphism; they were most closely related to those of Vibrio spp., Pseudomonas spp., and members of the family Enterobacteriaceae (72.4 to 97.1% homology). All 14 environmental resistant strains carried a point mutation in the GyrA QRDR at codon 83, leading to the substitution Ser-83->Ile (10 strains) or Ser-83->Arg. In addition, seven strains harbored a mutation in the ParC QRDR either at position 80 (five strains), generating a Ser-80->Ile (three strains) or Ser-80->Arg change, or at position 84, yielding a Glu-84->Lys modification. No amino acid alterations were discovered in the GyrB and ParE QRDRs. Double gyrA-parC missense mutations were associated with higher levels of quinolone resistance compared with the levels associated with single gyrA mutations. The most resistant strains probably had an additional mechanism(s) of resistance, such as decreased accumulation of the drugs. Our data suggest that, in mesophilic Aeromonas spp., as in other gram-negative bacteria, gyrase and topoisomerase IV are the primary and secondary targets for quinolones, respectively.

* Corresponding author. Mailing address: Laboratoire de Microbiologie, UFR des Sciences Pharmaceutiques, Université de Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France. Phone: 33 5 57 57 10 75. Fax: 33 5 56 90 90 72. E-mail: claudine.quentin{at}bacterio.u-bordeaux2.fr.

Antimicrobial Agents and Chemotherapy, February 2002, p. 350-359, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.350-359.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, L., Cagnon, C., Caumette, P., Duran, R. (2009). First Gene Cassettes of Integrons as Targets in Finding Adaptive Genes in Metagenomes. Appl. Environ. Microbiol. 75: 3823-3825 [Abstract] [Full Text]  
  • Roig, F. J., Llorens, A., Fouz, B., Amaro, C. (2009). Spontaneous Quinolone Resistance in the Zoonotic Serovar of Vibrio vulnificus. Appl. Environ. Microbiol. 75: 2577-2580 [Abstract] [Full Text]  
  • Picao, R. C., Poirel, L., Demarta, A., Silva, C. S. F., Corvaglia, A. R., Petrini, O., Nordmann, P. (2008). Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake. J Antimicrob Chemother 62: 948-950 [Abstract] [Full Text]  
  • Sanchez-Cespedes, J., Blasco, M. D., Marti, S., Alba, V., Alcalde, E., Esteve, C., Vila, J. (2008). Plasmid-Mediated QnrS2 Determinant from a Clinical Aeromonas veronii Isolate. Antimicrob. Agents Chemother. 52: 2990-2991 [Full Text]  
  • Hernould, M., Gagne, S., Fournier, M., Quentin, C., Arpin, C. (2008). Role of the AheABC Efflux Pump in Aeromonas hydrophila Intrinsic Multidrug Resistance. Antimicrob. Agents Chemother. 52: 1559-1563 [Abstract] [Full Text]  
  • Han, X. Y., Andrade, R. A. (2005). Brevundimonas diminuta infections and its resistance to fluoroquinolones. J Antimicrob Chemother 55: 853-859 [Abstract] [Full Text]  
  • Giraud, E., Blanc, G., Bouju-Albert, A., Weill, F.-X., Donnay-Moreno, C. (2004). Mechanisms of quinolone resistance and clonal relationship among Aeromonas salmonicida strains isolated from reared fish with furunculosis. J Med Microbiol 53: 895-901 [Abstract] [Full Text]  
  • Gibello, A., Porrero, M. C., Blanco, M. M., Vela, A. I., Liebana, P., Moreno, M. A., Fernandez-Garayzabal, J. F., Dominguez, L. (2004). Analysis of the gyrA Gene of Clinical Yersinia ruckeri Isolates with Reduced Susceptibility to Quinolones. Appl. Environ. Microbiol. 70: 599-602 [Abstract] [Full Text]  
  • Hurtle, W., Lindler, L., Fan, W., Shoemaker, D., Henchal, E., Norwood, D. (2003). Detection and Identification of Ciprofloxacin-Resistant Yersinia pestis by Denaturing High-Performance Liquid Chromatography. J. Clin. Microbiol. 41: 3273-3283 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»