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Antimicrobial Agents and Chemotherapy, February 2002, p. 360-366, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.360-366.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Cloning and Characterization of the Salmonella enterica Serovar Paratyphi B rma Gene, Which Confers Multiple Drug Resistance in Escherichia coli

Mahmoud. A. Yassien,1 Hosam E. Ewis,2 Chung-Dar Lu,2 and Ahmed T. Abdelal2*

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt,1 Department of Biology, Georgia State University, Atlanta, Georgia 303032

Received 17 August 2001/ Returned for modification 27 September 2001/ Accepted 29 October 2001

A genomic library from a strain of Salmonella enterica serovar Paratyphi B that exhibits multiple drug resistance (MDR) was constructed in Escherichia coli. Two of the recombinant plasmids, pNOR5 and pNOR5, conferred resistance only to fluoroquinolones in E. coli, whereas the third, pNCTR4, conferred the MDR phenotype. Sequence and subcloning analysis showed that it is the presence of RecA on the first two plasmids which confers resistance to fluoroquinolones in E. coli. A similar analysis established that the MDR phenotype conferred by pNCTR4 is due to a gene, rma (resistance to multiple antibiotics), which encodes a 13.5-kDa polypeptide. The derived sequence for Rma exhibits a high degree of similarity to those of a group of MarA-like activators that confer MDR in E. coli. A MalE-Rma fusion protein was purified to near homogeneity and was shown to interact with a DNA fragment carrying a MarA operator sequence. Furthermore, overexpression of rma in E. coli caused changes in the outer membrane protein profile that were similar to those reported for MarA. These results suggest that Rma might act as a transcriptional activator of the marA regulon.

* Corresponding author. Mailing address: College of Arts and Sciences, Georgia State University, P.O. Box 4038, Atlanta, GA 30302-4038. Phone: (404) 651-1410. Fax: (404) 651-4739. E-mail: aabdelal{at}gsu.edu.

Antimicrobial Agents and Chemotherapy, February 2002, p. 360-366, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.360-366.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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