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Antimicrobial Agents and Chemotherapy, February 2002, p. 511-513, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.511-513.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Analysis of Rifampin Resistance in Bacillus anthracis and Bacillus cereus

Amy J. Vogler, Joseph D. Busch, Stephanie Percy-Fine, Christine Tipton-Hunton, Kimothy L. Smith, and Paul Keim*

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640

Received 5 June 2001/ Returned for modification 27 August 2001/ Accepted 18 October 2001

Rifampin-resistant mutants were selected from UV-light-treated Bacillus cereus (20 mutants) and attenuated B. anthracis (23 mutants). In addition, spontaneous rifampin-resistant mutants were also isolated in attenuated B. anthracis (22 mutants). The rifampin resistance clusters of the rpoB gene were sequenced for all 65 mutants. Mutations associated with resistance were consistent with those from other bacteria, though two novel changes were observed. The spontaneous rate of resistance was estimated at 1.57 x 10-9 mutations/generation by a Luria-Delbrück fluctuation test.

* Corresponding author. Mailing address: Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640. Phone: (928) 523-1078. Fax: (928) 523-7500. E-mail Paul.Keim{at}nau.edu.

Antimicrobial Agents and Chemotherapy, February 2002, p. 511-513, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.511-513.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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