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Antimicrobial Agents and Chemotherapy, February 2002, p. 519-521, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.519-521.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Intramacrophage Passage of Mycobacterium tuberculosis and M. avium Complex Alters the Drug Susceptibilities of the Organisms as Determined by Intracellular Susceptibility Testing Using Macrophages and Type II Alveolar Epithelial Cells

Haruaki Tomioka,* Katsumasa Sato, Chiaki Sano, Keisuke Sano, and Toshiaki Shimizu

Department of Microbiology and Immunology, Shimane Medical University, Izumo, Shimane 693-8501, Japan

Received 30 July 2001/ Returned for modification 11 September 2001/ Accepted 5 November 2001

Mycobacterium tuberculosis and M. avium complex strains given intramacrophage passage (I-type) were compared with those cultured in a liquid medium (E-type) for their drug susceptibilities when they were replicating in Mono-Mac-6 macrophages or A-549 cells. Their intracellular susceptibilities to rifalazil, clarithromycin, and levofloxacin were decreased more in I-type organisms than in E-type organisms, except that their rifalazil susceptibility inside A-549 cells was markedly increased in I-type organisms.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Shimane Medical University, Izumo, Shimane 693-8501, Japan. Phone: 81 (853) 20-2146. Fax: 81 (853) 20-2145. E-mail: tomioka{at}shimane-med.ac.jp..

Antimicrobial Agents and Chemotherapy, February 2002, p. 519-521, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.519-521.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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