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Antimicrobial Agents and Chemotherapy, February 2002, p. 531-533, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.531-533.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sterol and Fatty Acid Composition of Candida lusitaniae Clinical Isolates

F. Peyron,1 A. Favel,1* R. Calaf,2 A. Michel-Nguyen,3 R. Bonaly,4 and J. Coulon4

Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire,1 Laboratoire de Biochimie Fondamentale et Clinique, Faculté de Pharmacie, 13385 Marseille,2 Laboratoire de Microbiologie, CHU Nord, 13015 Marseille,3 Laboratoire de Biochimie Microbienne, LCPME-UMR 7564, Faculté des Sciences Pharmaceutiques et Biologiques, 54001 Nancy, France4

Received 25 April 2001/ Returned for modification 21 May 2001/ Accepted 13 October 2001

The sterol and fatty acid compositions of four amphotericin B-resistant and of two amphotericin B-susceptible Candida lusitaniae clinical isolates were determined. A flow cytofluorometric susceptibility test (FCST) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. Compared to susceptible isolates, resistant ones showed a greatly reduced ergosterol content and changes in sterol composition, consistent with a defect in {Delta}8->7 isomerase. Within each group, no correlation between the sterol or fatty acid pattern or composition and both the degree of in vitro susceptibility and FCST MIC was found.

* Corresponding author. Mailing address: Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire, Faculté de Pharmacie, 27 Blvd. J. Moulin, 13005 Marseille Cedex 5, France. Phone: 33-4-91-83-56-37. Fax: 33-91-80-26-12. Email: Reglip{at}pharmacie.univ-mrs.fr.

Antimicrobial Agents and Chemotherapy, February 2002, p. 531-533, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.531-533.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miller, N. S., Dick, J. D., Merz, W. G. (2006). Phenotypic Switching in Candida lusitaniae on Copper Sulfate Indicator Agar: Association with Amphotericin B Resistance and Filamentation. J. Clin. Microbiol. 44: 1536-1539 [Abstract] [Full Text]  
  • Favel, A., Michel-Nguyen, A., Datry, A., Challier, S., Leclerc, F., Chastin, C., Fallague, K., Regli, P. (2004). Susceptibility of clinical isolates of Candida lusitaniae to five systemic antifungal agents. J Antimicrob Chemother 53: 526-529 [Abstract] [Full Text]  
  • Young, L. Y., Hull, C. M., Heitman, J. (2003). Disruption of Ergosterol Biosynthesis Confers Resistance to Amphotericin B in Candida lusitaniae. Antimicrob. Agents Chemother. 47: 2717-2724 [Abstract] [Full Text]  


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