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Antimicrobial Agents and Chemotherapy, February 2002, p. 561-565, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.561-565.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Inducible, but Not Constitutive, Resistance of Gonococci to Hydrophobic Agents Due to the MtrC-MtrD-MtrE Efflux Pump Requires TonB-ExbB-ExbD Proteins

Corinne Rouquette-Loughlin,1 Igor Stojiljkovic,1 Tara Hrobowski,1 Jacqueline T. Balthazar,1 and William M. Shafer1,2*

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322,1 Laboratories of Microbial Pathogenesis, Veterans Affairs Medical Center, Decatur, Georgia 300332

Received 6 April 2001/ Returned for modification 22 May 2001/ Accepted 25 October 2001

The MtrC-MtrD-MtrE efflux pump possessed by Neisseria gonorrhoeae is very similar to the MexA-MexB-OprM efflux pump of Pseudomonas aeruginosa. Because the antimicrobial resistance property afforded by the MexA-MexB-OprM efflux pump also requires the TonB protein, we asked whether a similar requirement exists for the gonococcal efflux pump. Unlike earlier studies with P. aeruginosa, we found that constitutive levels of gonococcal resistance to hydrophobic antimicrobial agents (i.e., Triton X-100 [TX-100]) did not require the TonB, ExbB, or ExbD protein. However, inducible levels of TX-100 resistance in gonococci had an absolute requirement for the TonB-ExbB-ExbD system, suggesting that such resistance in gonococci has an energy requirement above and beyond that required for constitutive pump activity.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322. Phone: (404) 728-7688. Fax: (404) 329-2210. E-mail: wshafer{at}emory.edu.

Antimicrobial Agents and Chemotherapy, February 2002, p. 561-565, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.561-565.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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