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Antimicrobial Agents and Chemotherapy, March 2002, p. 654-658, Vol. 46, No. 3
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.3.654-658.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ribosomal Mutations in Streptococcus pneumoniae Clinical Isolates

Antimicrobial Research Laboratory, National Public Health InstituteDepartments of,¹ Medicine,² Pediatrics,³ Ophthalmology, Turku University Central Hospital, Turku,⁴ Laboratory for Chlamydia and Bacterial Respiratory Infection, National Public Health Institute, Oulu, Finland,⁶ Centers for Disease Control and Prevention, Atlanta, Georgia⁵

Received 13 June 2001/ Returned for modification 3 October 2001/ Accepted 23 November 2001

Eleven clinical isolates of Streptococcus pneumoniae, isolated in Finland during 1996 to 2000, had an unusual macrolide resistance phenotype. They were resistant to macrolides and streptogramin B but susceptible, intermediate, or low-level resistant to lincosamides. No acquired macrolide resistance genes were detected from the strains. The isolates were found to have mutations in domain V of the 23S rRNA or ribosomal protein L4. Seven isolates had an A2059C mutation in two to four out of the four alleles encoding the 23S rRNA, two isolates had an A2059G mutation in two alleles, one isolate had a C2611G mutation in all four alleles, and one isolate had a ₆₉GTG₇₁-to-₆₉TPS₇₁ substitution in ribosomal protein L4.

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